Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

Citações e referências (163)

Invitrogen™

Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)

The heterobifunctional, crossslinker succinimidyl 3-(2-pyridyldithio)propionate (SPDP) enables one to covalently link two target groups. The succinimidyl ester chemically reacts withLeia mais

Número do catálogo	Quantity
S1531	100 mg

Número do catálogo S1531

Preço (BRL)

2.783,67

Adicionar ao carrinho

Quantity:

100 mg

Preço (BRL)

2.783,67

Adicionar ao carrinho

The heterobifunctional, crossslinker succinimidyl 3-(2-pyridyldithio)propionate (SPDP) enables one to covalently link two target groups. The succinimidyl ester chemically reacts with primary amines and after mild reduction of the dithiol, it can react form a disulfide with other thiols. Crosslinking reagents are useful for probing the spatial relationships and interactions within and between biomolecules.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Cell PermeabilityYes

DescriptionSPDP

FormSolid

Labeling MethodChemical Labeling

Molecular Weight (g/mol)312.36

PEGylatedNo

Quantity100 mg

Reactive MoietyNHS Ester, Pyridyldisulfide

SolubilityDMF, DMSO

Spacer Arm Length6.8 Å

Water SolubleNo

Chemical ReactivityAmine-Sulfhydryl

CleavableBy Thiols

Crosslinker TypeHeterobifunctional

FormatStandard

Product TypeCrosslinker

SpacerShort (<10 Å)

Unit SizeEach

Conteúdo e armazenamento

Upon receipt store at -20°C protected from light.

Frequently asked questions (FAQs)

Can you provide the shelf-life for Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP)?

Succinimidyl 3-(2-Pyridyldithio)Propionate (SPDP) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citações e referências (163)

Citações e referências

Abstract

Blockade of the galactose-binding sites of ricin by its linkage to antibody. Specific cytotoxic effects of the conjugates.

Authors:Thorpe PE,Ross WC,Brown AN,Myers CD,Cumber AJ,Foxwell BM,Forrester JT

Journal:European journal of biochemistry

PubMed ID:6608447

A method is described for preparing specific cytotoxic agents by linking intact ricin to antibodies in a manner that produces obstruction of the galactose-binding sites on the B chain of the toxin and so diminishes the capacity of the conjugate to bind non-specifically to cells. The conjugates were synthesised by ... More

Enhancement of the thrombolytic potency of plasminogen activators by conjugation with clot-specific monoclonal antibodies.

Authors:Dewerchin M, Collen D

Journal:Bioconjug Chem

PubMed ID:1790167

Detection of one attomole of [Arg8]-vasopressin by novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay).

Authors:Hashida S, Tanaka K, Yamamoto N, Uno T, Yamaguchi K, Ishikawa E

Journal:J Biochem (Tokyo)

PubMed ID:1778973

'One attomole of [Arg8]-vasopressin (AVP) was detected by a novel noncompetitive enzyme immunoassay (hetero-two-site complex transfer enzyme immunoassay). AVP was indirectly biotinylated using N-hydroxysuccinimidobiotin and trapped onto an anti-AVP IgG-coated polystyrene ball. After washing, biotinylated AVP was eluted from the polystyrene ball with HCl and was reacted with 2,4-dinitrophenyl-fluorescein disulfide-bovine ... More

Activation of methotrexate-alpha-alanine by carboxypeptidase A-monoclonal antibody conjugate.

Authors:Haenseler E, Esswein A, Vitols KS, Montejano Y, Mueller BM, Reisfeld RA, Huennekens FM

Journal:Biochemistry

PubMed ID:1731945

'Carboxypeptidase A (CP-A) and monoclonal antibody KS1/4 directed against an antigen on human lung adenocarcinoma cells (UCLA-P3) were derivatized by treatment with succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate and N-succinimidyl 3-(2-pyridyldithio)propionate, respectively. The derivatized proteins were reacted to produce thioether-linked enzyme-antibody conjugates. Sequential HPLC size-exclusion and DEAE chromatography separated the conjugate preparation from unreacted ... More

Selective destruction of nerve growth factor receptor-bearing cells in vitro using a hybrid toxin composed of ricin A chain and a monoclonal antibody against the nerve growth factor receptor.

Authors:DiStefano PS, Schweitzer JB, Taniuchi M, Johnson EM

Journal:J Cell Biol

PubMed ID:2993314

'A hybrid toxin composed of ricin A chain and a monoclonal antibody directed against the rat nerve growth factor (NGF) receptor (192-IgG) was prepared using the heterobifunctional cross-linking agent N-succinimidyl-3-(2-pyridyldithio)-propionate and purified by affinity chromatography. Characterization studies showed that the hybrid, 192-s-s-A, displaced bound 125I-labeled 192-IgG from rat superior cervical ... More

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