Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)
Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)
Invitrogen™

Streptavidin, Alexa Fluor™ 610—R-Phycoerythrin Conjugate (Alexa Fluor™ 610—R-Phycoerythrin Streptavidin)

The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ∼496,565/627 and is widely used toLeia mais
Have Questions?
Número do catálogoQuantity
S20982
conhecido também como S-20982
100 μL
Número do catálogo S20982
conhecido também como S-20982
Preço (BRL)
-
Quantity:
100 μL
The streptavidin, Alexa Fluor™ 610-R-phycoerythrin streptavidin tandem conjugate has excitation and emission maxima at ∼496,565/627 and is widely used to detect biotinylated probes by flow cytometry. Not only are the Alexa Fluor™ 610 conjugates of R-PE are more fluorescent than the commercially available Texas Red™ conjugates of R-PE, the fluorescent emission of Alexa Fluor™ 610-R-PE tandem conjugates is shifted to somewhat longer wavelengths than is the emission of Texas Red™-R-PE conjjgates, resulting in better separation from the emission of R-PE.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Concentration1 mg/mL
Detection MethodFluorescence
Excitation/Emission488 or 561/630
Flow Cytometer Laser Lines488 or 561
For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis
Label or DyePE-Alexa Fluor 610
Product TypeStreptavidin Conjugate (Fluorescent)
Quantity100 μL
Shipping ConditionWet Ice
ConjugatePE-Alexa Fluor 610
FormSolution
Product LineAlexa Fluor
Unit SizeEach
Conteúdo e armazenamento
Contains 1 vial of streptavidin R-PE-Alexa Fluor™ 610 conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (2)

Citações e referências
Abstract
Authors:
Journal:
PubMed ID:18258751
Nucleoside salvage pathway kinases regulate hematopoiesis by linking nucleotide metabolism with replication stress.
Authors:Austin WR, Armijo AL, Campbell DO, Singh AS, Hsieh T, Nathanson D, Herschman HR, Phelps ME, Witte ON, Czernin J, Radu CG,
Journal:J Exp Med
PubMed ID:23148236
'Nucleotide deficiency causes replication stress (RS) and DNA damage in dividing cells. How nucleotide metabolism is regulated in vivo to prevent these deleterious effects remains unknown. In this study, we investigate a functional link between nucleotide deficiency, RS, and the nucleoside salvage pathway (NSP) enzymes deoxycytidine kinase (dCK) and thymidine ... More