Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)

Citações e referências (1)

Invitrogen™

Streptavidin, Alexa Fluor™ 750—Allophycocyanin Conjugate (Alexa Fluor™ 750—Allophycocyanin Streptavidin)

The streptavidin, Alexa Fluor™ 750-allophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at ∼650/779 and is widely usedLeia mais

Número do catálogo	Quantity
S21008 conhecido também como S-21008	100 μL

Número do catálogo S21008

conhecido também como S-21008

Preço (BRL)

Quantity:

100 μL

The streptavidin, Alexa Fluor™ 750-allophycocyanin (APC) streptavidin tandem conjugate has excitation and emission maxima at ∼650/779 and is widely used to detect biotinylated probes by flow cytometry in multicolor applications following excitation at 633 nm or 647 nm.

View a selection guide for all Alexa Fluor™ streptavidin and avidin conjugates.

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Concentration1 mg/mL

Detection MethodFluorescence

Excitation/Emission633 or 635/775

Flow Cytometer Laser Lines633 or 635

For Use With (Equipment)Flow Cytometry, Immunoassays, Histochemical Applications, Blot Analysis

Label or DyeAPC-Alexa Fluor 750

Product TypeStreptavidin Conjugate (Fluorescent)

Quantity100 μL

Shipping ConditionWet Ice

ConjugateAPC-Alexa Fluor 750

FormSolution

Product LineAlexa Fluor

Unit SizeEach

Conteúdo e armazenamento

Contains 1 vial of streptavidin APC-Alexa Fluor™ 750 conjugate (1 mg/mL).

Store in refrigerator (2–8°C) and protect from light.

Frequently asked questions (FAQs)

Can I use my tandem dye antibody (e.g., Alexa Fluor 610-R-phycoerythrin) for microscopy?

Tandem dyes are not recommended for imaging applications. Both R-PE or APC tend to photobleach quickly and cannot be archived. Any unfolding of the phycobiliprotein would result in loss of fluorescence. They work great in flow cytometry applications.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

How should I store my allophycocyanin (APC)-conjugated antibody?

Allophycocyanin-conjugated antibodies must be stored at 2-8 degrees C. Antibodies conjugated to phycobiliproteins (APC, R-PE) must never be frozen. Once frozen, even briefly, they are no longer usable.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (1)

Citações e referências

Abstract

Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: fluorescence of the dyes and their bioconjugates.

Authors:Berlier JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP

Journal:J Histochem Cytochem

PubMed ID:14623938

'Amine-reactive N-hydroxysuccinimidyl esters of Alexa Fluor fluorescent dyes with principal absorption maxima at about 555 nm, 633 nm, 647 nm, 660 nm, 680 nm, 700 nm, and 750 nm were conjugated to antibodies and other selected proteins. These conjugates were compared with spectrally similar protein conjugates of the Cy3, Cy5, ... More