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Citações e referências (6)
Invitrogen™
SNARF™-5F 5-(and-6)-Carboxylic Acid
Cell-impermeant ratiometric pH indicator.
| Número do catálogo | Quantity |
|---|---|
| S23922 conhecido também como S-23922 | 1 mg |
Número do catálogo S23922
conhecido também como S-23922
Preço (BRL)
-
Quantity:
1 mg
The cell-impermeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid (pKa of ∼7.2) exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
IndicatorpH Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Application)pH Indicator
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineSNARF
Product TypepH Indicator
Unit SizeEach
Conteúdo e armazenamento
Store at room temperature and protect from light.
Citações e referências (6)
Citações e referências
Abstract
Characterization of dual-wavelength seminaphthofluorescein and seminapthorhodafluor dyes for pH sensing under high hydrostatic pressures.
Journal:Anal Biochem
PubMed ID:17274941
'Hydrostatic pressure is an important physical parameter in biology, with pressures in the few-hundred-atm range having significant effects on cellular morphology, metabolism, and viability. To ensure valid results when studying pressure effects using fluorescence spectroscopy and imaging methods, metabolic probes need to be characterized for high-pressure use. Of interest is
Fluorescent indicators for intracellular pH.
Journal:Chem Rev
PubMed ID:19831417
This review is about intracellular pH sensors, includingsmall fluorescent organic molecules, nanoparticles, andfluorescent proteins, e.g., GFP. It focuses on their preparations, photophysical properties, and advantages/disadvantagesfor intracellular pH measurements. The discussion is limitedto fluorescent indicators that have been applied to measureintracellular pH values since 1980.
Synthesis and photophysical properties of new fluorinated benzo[c]xanthene dyes as intracellular pH indicators.
Journal:Bioorg Med Chem Lett
PubMed ID:11677123
Two new fluorinated benzo[c]xanthene dyes were synthesized by reaction of fluorinated 1,6-dihydroxynaphthalenes with 2,4- (and 2,5)-dicarboxy-3'-dimethylamino-2'-hydroxybenzophenone. The two critical fluorinated 1,6-dihydroxynaphthalene intermediates were prepared via a regioselective route. The fluorinated benzo[c]xanthene dyes exhibit desired lower pK(a) values (6.4 and 7.2, respectively) than their parent compound (pK(a)=7.5) while the pH-dependent dual-emission
Using LysoSensor Yellow/Blue DND-160 to sense acidic pH under high hydrostatic pressures.
Journal:Anal Biochem
PubMed ID:18976626
LysoSensor Yellow/Blue DND-160, a dual-wavelength fluorophore commonly used for sensing pH in acidic organelles, possesses solvatochromic behavior believed to originate from an intramolecular charge transfer (ICT). Given this, we investigated whether DND-160 can be used for acidic pH sensing under hydrostatic pressures up to 510 atm, a range suitable for
The chloride transporter Na(+)-K(+)-Cl- cotransporter isoform-1 contributes to intracellular chloride increases after in vitro ischemia.
Journal:J Neurosci
PubMed ID:16452663
Ischemic episodes in the CNS cause significant disturbances in neuronal ionic homeostasis. To directly measure changes in intracellular Cl- concentration ([Cl-]i) during and after ischemia, we used Clomeleon, a novel ratiometric optical indicator for Cl-. Hippocampal slices from adult transgenic mice expressing Clomeleon in hippocampal neurons were subjected to 8