SYPRO™ Protein Gel Stains

Citações e referências (60)

Invitrogen™

SYPRO™ Protein Gel Stains

Name: SYPRO™ Protein Gel Stains
SKU: S6654

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE).

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Número do catálogo	Color	Quantity
S6654 conhecido também como S-6654	Red	10 x 50 μL
S6650	Orange	500 μL
S6651	Orange	10 x 50 μL
S12010	Tangerine	500 μL
S6653	Red	500 μL
S12001	Ruby	200 mL
S12000	Ruby	1 L
S21900	Ruby	5 L

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Número do catálogo S6654

conhecido também como S-6654

Preço (BRL)

Color:

Red

Quantity:

10 x 50 μL

Request bulk or custom format

SYPRO protein gel stains are sensitive, easy-to-use fluorescent stains for total proteins separated by polyacrylamide gel electrophoresis (PAGE). Stained proteins can be viewed with a standard UV or blue-light transilluminator, or imaging equipment containing the appropriate filters or laser. SYPRO protein gel stains offer many advantages over Coomassie Blue and silver staining, including a fast, one-step staining protocol requiring no destaining, a linear dynamic range over three orders of magnitude, and very little protein-to-protein variation in staining.

SYPRO Ruby Protein Gel Stain features include:

Convenient—ready-to-use formulation
High sensitivity—detection to 0.25 to 1 ng
Effective staining for 1D and 2D gels
Can be multiplexed with other gel stains such as Pro-Q Diamond phosphoprotein gel stain or Pro-Q Emerald 300 glycoprotein gel stain
Compatible with subsequent analysis of proteins by Edman-based sequencing or mass spectrometry

SYPRO Orange and SYPRO Red protein gel stain features include:

Sensitive—detection to 4 to 8 ng
Fast—rapid staining time of 10–60 min
Best for 1D gels
SYPRO Orange: Ex: 300, 470 nm, Em: 570 nm
SYPRO Red: Ex: 300, 550 nm, Em: 630 nm

SYPRO Tangerine Protein Gel Stain features include:

Sensitive—detection to 4 to 8 ng
Fast—rapid staining time of 10 to 60 minutes
Best for 1D gels
Does not require fixation with acetic acid
Compatible with subsequent gel staining, western blotting, zymography, electroelution or mass spectrometry

For Research Use Only. Not for use in diagnostic procedures.

Especificações

Concentration5000X in DMSO

Detection LocationIn-Gel Detection

Detection MethodFluorescence

Excitation/Emission300, 550/630 nm

Quantity10 x 50 μL

Shipping ConditionRoom Temperature

Target MoleculeProtein

ColorRed

Label or DyeSYPRO Red

Product LineSYPRO

Product TypeProtein Gel Stain

Unit SizeEach

Conteúdo e armazenamento

Store at room temperature and protect from light.

Frequently asked questions (FAQs)

If I increase the final concentration of my SYPRO Orange or SYPRO Red staining solution above 1X, can I increase the signal of my stained proteins?

No. Dye concentrations higher than 1X do not give better detection. Instead, background fluorescence increases and, as the dye concentration increases, the dye becomes self-quenching and the signal actually decreases.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I dry my SYPRO Ruby, SYPRO Orange, or SYPRO Red stained gels?

Gels stained with SYPRO dyes can be dried between sheets of cellophane, although there is sometimes a slight decrease in sensitivity. If the gels are dried onto paper, the light will scatter and the sensitivity will decrease. Other plastics are not recommended, as the plastic typically used is not transparent to UV light.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I pre-stain proteins with SYPRO Ruby, SYPRO Orange or SYPRO Red Protein Gel Stains and then run them through a gel?

No. Loading solutions contain so much SDS that SYPRO Ruby, SYPRO Orange and SYPRO Red dyes simply localize in the free SDS and bind very little of the proteins. Proteins can be covalently pre-labeled with ATTO-TAG CBQCA (Cat. No. A2333), DDAO succinimidyl ester (Cat. No. C34553) or TAMRA-succinimidyl ester (Cat. No. C2211) dyes, or the TC-FLAsH Expression Analysis Detection Kits (Cat. No. A10067 for orange fluorescence, Cat. No. A10068 for red fluorescence) prior to electrophoresis without affecting protein migration through the gel.

SYPRO Orange or SYPRO Red Protein Gel Stain can be diluted 5000-fold into the cathode (upper) buffer tank to stain proteins during electrophoresis without affecting migration. The problem with doing this is that there is considerable background fluorescence in the gels from the dye interacting with SDS. This can be reduced after electrophoresis by destaining in 7.5% acetic acid for 15-60 minutes. This method also results in poorer protein sensitivity than the standard post-staining method, requires the same amount of time before the gel can be imaged, and contaminates the electrophoresis apparatus.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Citações e referências (60)

Citações e referências

Abstract

Design and characterization of a compact dual channel virus counter.

Authors:Stoffel CL,Kathy RF,Rowlen KL

Journal:Cytometry. Part A : the journal of the International Society for Analytical Cytology

PubMed ID:15830378

Adenosine to inosine editing by ADAR2 requires formation of a ternary complex on the GluR-B R/G site.

Authors:Jaikaran DC, Collins CH, MacMillan AM

Journal:J Biol Chem

PubMed ID:12163487

'RNA editing by members of the ADAR (adenosine deaminase that acts on RNA) enzyme family involves hydrolytic deamination of adenosine to inosine within the context of a double-stranded pre-mRNA substrate. Editing of the human GluR-B transcript is catalyzed by the enzyme ADAR2 at the Q/R and R/G sites. We have ... More

Defining the SNARE complex binding surface of alpha-SNAP: implications for SNARE complex disassembly.

Authors:Marz KE, Lauer JM, Hanson PI

Journal:J Biol Chem

PubMed ID:12730228

'N-Ethylmaleimide-sensitive factor (NSF) and its adaptor protein alpha-soluble NSF attachment protein (alpha-SNAP) sustain membrane trafficking by disassembling soluble NSF attachment protein receptor (SNARE) complexes that form during membrane fusion. To better understand the role of alpha-SNAP in this process, we used site-directed mutagenesis to identify residues in alpha-SNAP that interact ... More

Involvement of DnaE, the second replicative DNA polymerase from Bacillus subtilis, in DNA mutagenesis.

Authors:Le Chatelier E, Bécherel OJ, d'Alençon E, Canceill D, Ehrlich SD, Fuchs RP, Jannière L

Journal:J Biol Chem

PubMed ID:14593098

'In a large group of organisms including low G + C bacteria and eukaryotic cells, DNA synthesis at the replication fork strictly requires two distinct replicative DNA polymerases. These are designated pol C and DnaE in Bacillus subtilis. We recently proposed that DnaE might be preferentially involved in lagging strand ... More

Dynamics of myo1c (myosin-ibeta ) lipid binding and dissociation.

Authors:Tang N, Lin T, Ostap EM

Journal:J Biol Chem

PubMed ID:12221091

'Myosin-I is the single-headed member of the myosin superfamily that associates with lipid membranes. Biochemical experiments have shown that myosin-I membrane binding is the result of electrostatic interactions between the basic tail domain and acidic phospholipids. To better understand the dynamics of myosin-I membrane association, we measured the rates of ... More

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