Vybrant™ Cell Adhesion Assay Kit
Vybrant™ Cell Adhesion Assay Kit
Invitrogen™

Vybrant™ Cell Adhesion Assay Kit

The Vybrant™ Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for aLeia mais
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Número do catálogoQuantity
V131811 kit
Número do catálogo V13181
Preço (BRL)
3.952,44
1 kit
Adicionar ao carrinho
Quantity:
1 kit
Preço (BRL)
3.952,44
1 kit
Adicionar ao carrinho
The Vybrant™ Cell Adhesion Assay Kit is a fast and sensitive assay for measuring cell-cell or cell-surface adhesion for a variety of cell types. In this assay, cells are labeled with calcein AM and allowed to adhere. After removal of nonadherent cells, calcein fluorescence is used to calculate the number of adherent cells.
For Research Use Only. Not for use in diagnostic procedures.
Especificações
Detection MethodFluorescence
Dye TypeOther Label(s) or Dye(s)
Format96-well plate
Quantity1 kit
Shipping ConditionRoom Temperature
For Use With (Application)Cell Adhesion Assay
For Use With (Equipment)Microplate Reader
Product LineVybrant
Product TypeStain
Unit Size1 kit
Conteúdo e armazenamento
Store in freezer -5°C to -30°C and protect from light.

Frequently asked questions (FAQs)

What are the fluorescence excitation/emission maxima for the Calcein AM and SYTOX Green nucleic acid stain provided in the Vybrant Cell Adhesion Assay Kit?


Calcein AM: Ex/Em = 494 nm/517 nm
SYTOX Green nucleic acid stain: Ex/Em = 504 nm/523 nm

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citações e referências (33)

Citações e referências
Abstract
Effect of C-reactive protein on gene expression in vascular endothelial cells.
Authors:Wang Q, Zhu X, Xu Q, Ding X, Chen YE, Song Q,
Journal:Am J Physiol Heart Circ Physiol
PubMed ID:15591095
'C-reactive protein (CRP) is significantly associated with the risk of ischemic cardiovascular disease in epidemiological studies. To explore if CRP has a functional role, we investigated its effect on the gene expression profile of vascular endothelial cells. Human vascular endothelial cells (human umbilical vein endothelial cells and human aortic endothelial ... More
Atherogenic lipids induce adhesion of human coronary artery smooth muscle cells to macrophages by up-regulating chemokine CX3CL1 on smooth muscle cells in a TNFalpha-NFkappaB-dependent manner.
Authors:Barlic J, Zhang Y, Murphy PM,
Journal:J Biol Chem
PubMed ID:17456471
'Recent genetic evidence has implicated the adhesive chemokine CX3CL1 and its leukocyte receptor CX3CR1 in atherosclerosis. We previously proposed a mechanism involving foam cell anchorage to vascular smooth muscle cells because: 1) CX3CL1 and CX3CR1 are expressed by both cell types in mouse and human atherosclerotic lesions; 2) foam cells ... More
ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells.
Authors:Li X, Krawetz R, Liu S, Meng G, Rancourt DE,
Journal:Hum Reprod
PubMed ID:19056770
'Efficient slow freezing protocols within serum-free and feeder-free culture systems are crucial for the clinical application of human embryonic stem (hES) cells. Frequently, however, hES cells must be cryopreserved as clumps when using conventional slow freezing protocols, leading to lower survival rates during freeze-thaw and limiting their recovery and growth ... More
CXCL16 signals via Gi, phosphatidylinositol 3-kinase, Akt, I kappa B kinase, and nuclear factor-kappa B and induces cell-cell adhesion and aortic smooth muscle cell proliferation.
Authors:Chandrasekar B, Bysani S, Mummidi S,
Journal:J Biol Chem
PubMed ID:14625285
'CXCL16, a recently discovered transmembrane chemokine, is expressed in human aortic smooth muscle cell (ASMC). It facilitates uptake of low density lipoproteins by macrophages, resulting in foam cell formation. However, it is not known whether ASMC express CXCR6, the receptor for CXCL16, or whether CXCL16 affects ASMC biology. To dissect ... More
Targeting PKC in multiple myeloma: in vitro and in vivo effects of the novel, orally available small-molecule inhibitor enzastaurin (LY317615.HCl).
Authors:Podar K, Raab MS, Zhang J, McMillin D, Breitkreutz I, Tai YT, Lin BK, Munshi N, Hideshima T, Chauhan D, Anderson KC,
Journal:Blood
PubMed ID:17023575
'In multiple myeloma (MM) protein kinase C (PKC) signaling pathways have been implicated in cell proliferation, survival, and migration. Here we investigated the novel, orally available PKC-inhibitor enzastaurin for its anti-MM activity. Enzastaurin specifically inhibits phorbol ester-induced activation of PKC isoforms, as well as phosphorylation of downstream signaling molecules MARCKS ... More