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Citations & References (185)
Invitrogen™
Lipofectamine™ 2000 Transfection Reagent
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety ofRead more
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| Catalog Number | Quantity |
|---|---|
| 11668019 | 1.5 mL |
| 11668500 | 15 mL |
| 11668027 | 0.75 mL |
| 11668030 | 0.3 mL |
Catalog number 11668019
Price (CLP)
834.775
Each
Quantity:
1.5 mL
Price (CLP)
834.775
Each
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine™ 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
Green FeaturesSustainable packaging
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine
Product TypeTransfection Reagent
Quantity1.5 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Contains one vial (1.5 ml) Lipofectamine™ 2000 Reagent. Store at 2–8°C. Do not freeze.
Frequently asked questions (FAQs)
I transfected GFP into cells using Lipofectamine 2000 and saw a light granular orange background fluorescence. What could be causing this?
I accidentally left my lipid reagent at room temperature. Can I still use it?
Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA?
Can I use Lipofectamine 2000 to co-transfect plasmids and siRNA?
For how long is the Lipofectamine 2000:DNA complex stable?
Citations & References (185)
Citations & References
Abstract
Role of tyrosine kinase Jak2 in prolactin-induced differentiation and growth of mammary epithelial cells.
Journal:J Biol Chem
PubMed ID:11821424
Genetic studies in mice have established a critical role for prolactin receptors and transcription factor Stat5 in mammary gland differentiation. However, the enzymatic coupling between prolactin receptors and Stat5 in this process has not been established. In addition to Jak2, several other tyrosine kinases reportedly also are associated with prolactin
Confirmation by FRET in individual living cells of the absence of significant amyloid beta -mediated caspase 8 activation.
Journal:Proc Natl Acad Sci U S A
PubMed ID:12409609
When cells are exposed to death-inducing molecules such as tumor necrosis factor-alpha or Fas, caspase 8 is activated and cleaves an apoptotic facilitator, Bid, that is a member of the Bcl-2 family. After additional modification, the C-terminal moiety of Bid is translocated to the mitochondria and induces the release of
AMP-activated Kinase Inhibits the Epithelial Na+ Channel through Functional Regulation of the Ubiquitin Ligase Nedd4-2.
Journal:J Biol Chem
PubMed ID:16844684
'We recently found that the metabolic sensor AMP-activated kinase (AMPK) inhibits the epithelial Na(+) channel (ENaC) through decreased plasma membrane ENaC expression, an effect requiring the presence of a binding motif in the cytoplasmic tail of the beta-ENaC subunit for the ubiquitin ligase Nedd4-2. To further examine the role of
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19307578
'Nucleic acid reagents, including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammalian cells and are promising starting points for the development of new therapeutic agents. Realizing their full potential, however, requires nucleic acid delivery reagents that are simple to prepare, effective across many
Dynamic fluorescent imaging of human immunodeficiency virus type 1 gag in live cells by biarsenical labeling.
Journal:J Virol
PubMed ID:15767407
'Human immunodeficiency virus type 1 (HIV-1) Gag is the primary structural protein of the virus and is sufficient for particle formation. We utilized the recently developed biarsenical-labeling method to dynamically observe HIV-1 Gag within live cells by adding a tetracysteine tag (C-C-P-G-C-C) to the C terminus of Gag in both