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Citations & References (55)
Invitrogen™
Lipofectamine™ 2000 Transfection Reagent
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety ofRead more
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| Catalog Number | Quantity |
|---|---|
| 11668500 | 15 mL |
| 11668019 | 1.5 mL |
| 11668027 | 0.75 mL |
| 11668030 | 0.3 mL |
Catalog number 11668500
Price (CLP)
8.556.537
Each
Quantity:
15 mL
Price (CLP)
8.556.537
Each
Lipofectamine™ 2000 Transfection Reagent is a versatile transfection reagent that has been shown to effectively transfect the widest variety of adherent and suspension cell lines. Researchers use Lipofectamine™ 2000 Reagent for siRNA- and shRNA-based gene knockdown experiments, as well as for gene expression studies.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
With Lipofectamine™ 2000 Transfection Reagent, you'll get:
• Exceptional transfection efficiency in the broadest range of cell lines and the highest levels of recombinant protein expression (see table)
• Superior performance for co-transfection of siRNA and plasmid DNA
• Proven efficacy in the presence of serum—eliminates the need to change media following transfection
• Reliable performance for high-throughput applications
• The best choice for establishing stable cell lines
A high-performance transfection reagent for gene expression and gene silencing
Lipofectamine™ 2000 Transfection Reagent works effectively with all common cell lines as well as with many challenging ones, and can be used in media with or without serum. For gene silencing, Lipofectamine™ 2000 Transfection Reagent's high-efficiency transfections provide the high levels of gene knockdown needed to produce convincing results. Lipofectamine™ 2000 Transfection Reagent is the number one choice for co-transfection, given its effectiveness for transfecting both siRNA and plasmid DNA. Lipofectamine™ 2000 Transfection Reagent is easy to use—simply mix with nucleic acid and add to cell culture.
Ideal for high-throughput work
Simplicity and speed combined with high transfection efficiency make Lipofectamine™ 2000 Transfection Reagent ideal for transient protein expression or high-throughput RNAi transfections. Transfection conditions can be easily established for automated or robotic systems used in such applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
Green FeaturesSustainable packaging
High-throughput CompatibilityHigh-throughput Compatible
Product LineLipofectamine
Product TypeTransfection Reagent
Quantity15 mL
Serum CompatibleYes
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
Cell TypeEstablished Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Format6-well Plate, 12-well Plate, 24-well Plate, 48-well Plate, 96-well Plate, Flasks
Sample TypePlasmid DNA, Synthetic siRNA, RNAi Plasmids (shRNA, miR)
Transfection TechniqueLipid-based Transfection
Unit SizeEach
Contents & Storage
Contains one bottle (15 mL) Lipofectamine™ 2000 Reagent. Store at 2–8°C. Do not freeze.
Frequently asked questions (FAQs)
I transfected GFP into cells using Lipofectamine 2000 and saw a light granular orange background fluorescence. What could be causing this?
I accidentally left my lipid reagent at room temperature. Can I still use it?
Can I use Lipofectamine RNAiMAX to co-transfect siRNA with plasmid DNA?
Can I use Lipofectamine 2000 to co-transfect plasmids and siRNA?
For how long is the Lipofectamine 2000:DNA complex stable?
Citations & References (55)
Citations & References
Abstract
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T
Mammalian cell penetration, siRNA transfection, and DNA transfection by supercharged proteins.
Journal:Proc Natl Acad Sci U S A
PubMed ID:19307578
'Nucleic acid reagents, including small interfering RNA (siRNA) and plasmid DNA, are important tools for the study of mammalian cells and are promising starting points for the development of new therapeutic agents. Realizing their full potential, however, requires nucleic acid delivery reagents that are simple to prepare, effective across many
Amino acid residues critical for endoplasmic reticulum export and trafficking of platelet-activating factor receptor.
Journal:J Biol Chem
PubMed ID:20007715
'Several residues are conserved in the transmembrane domains (TMs) of G-protein coupled receptors. Here we demonstrate that a conserved proline, Pro(247), in TM6 of platelet-activating factor receptor (PAFR) is required for endoplasmic reticulum (ER) export and trafficking after agonist-induced internalization. Alanine-substituted mutants of the conserved residues of PAFRs, including P247A,
Caveolin regulates endocytosis of the muscle repair protein, dysferlin.
Journal:J Biol Chem
PubMed ID:18096699
'Dysferlin and Caveolin-3 are plasma membrane proteins associated with muscular dystrophy. Patients with mutations in the CAV3 gene show dysferlin mislocalization in muscle cells. By utilizing caveolin-null cells, expression of caveolin mutants, and different mutants of dysferlin, we have dissected the site of action of caveolin with respect to dysferlin
Functional analysis of FSP27 protein regions for lipid droplet localization, caspase-dependent apoptosis, and dimerization with CIDEA.
Journal:Am J Physiol Endocrinol Metab
PubMed ID:19843876
'The adipocyte-specific protein FSP27, also known as CIDEC, is one of three cell death-inducing DFF45-like effector (CIDE) proteins. The first known function for CIDEs was promotion of apoptosis upon ectopic expression in mammalian cells. Recent studies in endogenous settings demonstrated key roles for CIDEs in energy metabolism. FSP27 is a