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Catalog Number | Unit Size | Price (CLP) | Availability | Quantity | |
---|---|---|---|---|---|
11965092 also known as 11965-092 | 500 mL | Price: 29.198 Your price: | *** | ||
11965118 also known as 11965-118 | 10 x 500 mL | Price: 251.351 Your price: | *** | ||
11965084 | 1,000 mL | Contact Us › | *** | - | |
11965126 | 6 x 1,000 mL | Contact Us › | *** | - | |
11965167 also known as 11965-167 | 5 L | Price: 298.198 Your price: | *** | ||
11965175 also known as 11965-175 | 10 L | Contact Us › | *** | - |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With | Without |
• High Glucose | • Sodium Pyruvate |
• L-glutamine | • HEPES |
• Phenol Red |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.