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Catalog Number | Unit Size | Price (CLP) | Availability | Quantity | |
---|---|---|---|---|---|
11995123 also known as 11995-123 | 10 L | Contact Us › | *** | - | |
11995065 | 500 mL | Price: 29.432 Your price: | *** | ||
11995073 also known as 11995-073 | 10 x 500 mL | Price: 253.153 Your price: | *** | ||
11995040 | 1,000 mL | Price: 47.523 Your price: | *** | ||
11995081 | 6 x 1,000 mL | Price: 243.243 Your price: | *** | ||
11995115 also known as 11995-115 | 5 L | Contact Us › | *** | - | |
41966161 | 10 L | Contact Us › | *** | - |
DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
With | Without |
• High Glucose | • HEPES |
• L-glutamine | |
• Phenol Red | |
• Sodium Pyruvate |
The complete formulation is available.
Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.