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Catalog Number | Unit Size | Price (CLP) | Availability | Quantity | |
---|---|---|---|---|---|
15593031 | 100 mL | Price: 178.378 Your price: | *** | ||
15593049 also known as 15593-049 | 400 mL | Price: 489.730 Your price: | *** |
UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.
Using Phenol:Chloroform:Isoamyl Alcohol
When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.
Performance and quality testing
The appearance of the solution is evaluated at room temperature. No RNase or DNase activity is detected.