EGS (ethylene glycol bis(succinimidyl succinate))

Citations & References (5)

Thermo Scientific™

EGS (ethylene glycol bis(succinimidyl succinate))

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can beRead more

Catalog Number	Quantity
21565	1 g

Catalog number 21565

Price (CLP)

286.363

Add to cart

Quantity:

Request bulk or custom format

Price (CLP)

286.363

Add to cart

Thermo Scientific Pierce EGS is a crosslinker that contains amine-reactive NHS-ester ends around a 12-atom spacer arm, which can be cleaved by treatment with hydroxylamine at pH 8.5.

Features of the EGS crosslinker:

• Reactive groups: NHS ester (both ends)
• Reactive towards: amino groups (primary amines)
• Water-insoluble (dissolve first in DMF or DMSO); compare to Sulfo-EGS
• Membrane-permeable, allowing for intracellular crosslinking
• Crosslinks formed are reversible at pH 8.5 using hydroxylamine for 3 to 6 hours at 37°C
• Lactose dehydrogenase retained 60% of its activity after reversible crosslinking with EGS

Product References:
Crosslinker Application Guide -- search for recent literature references for this product

Related Products
EGS (ethylene glycol bis(succinimidyl succinate))

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell PermeabilityYes

DescriptionEGS

FormPowder

Labeling MethodChemical Labeling

Molecular Weight (g/mol)456.36

PEGylatedNo

Product LinePierce

Quantity1 g

Reactive MoietyNHS Ester

Shipping ConditionAmbient

SolubilityDMF, DMSO

Spacer Arm Length16.1 Å

Water SolubleNo

Chemical ReactivityAmine-Amine

CleavableBy Hydroxylamine

Crosslinker TypeHomobifunctional

FormatStandard

Product TypeCrosslinker

SpacerMedium (10 to 30 Å)

Unit SizeEach

Contents & Storage

Upon receipt store at 4°C.

Frequently asked questions (FAQs)

Can you provide the shelf-life for EGS (ethylene glycol bis(succinimidyl succinate))?

EGS (ethylene glycol bis(succinimidyl succinate)) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (5)

Citations & References

Abstract

Oligomerization of the mitochondrial protein voltage-dependent anion channel is coupled to the induction of apoptosis.

Authors:Keinan N, Tyomkin D, Shoshan-Barmatz V

Journal:Mol Cell Biol

PubMed ID:20937774

'Accumulating evidence implicates that the voltage-dependent anion channel (VDAC) functions in mitochondrion-mediated apoptosis and as a critical player in the release of apoptogenic proteins, such as cytochrome c, triggering caspase activation and apoptosis. The mechanisms regulating cytochrome c release and the molecular architecture of the cytochrome c-conducting channel remain unknown. ... More

Structure-based analysis of VDAC1 protein: defining oligomer contact sites.

Authors:Geula S, Naveed H, Liang J, Shoshan-Barmatz V

Journal:J Biol Chem

PubMed ID:22117062

'The outer mitochondrial membrane protein, the voltage-dependent anion channel (VDAC), is increasingly implicated in the control of apoptosis. Oligomeric assembly of VDAC1 was shown to be coupled to apoptosis induction, with oligomerization increasing substantially upon apoptosis induction and inhibited by apoptosis blockers. In this study, structure- and computation-based selection of ... More

Integrated genome-wide analysis of transcription factor occupancy, RNA polymerase II binding and steady-state RNA levels identify differentially regulated functional gene classes.

Authors:Mokry M, Hatzis P, Schuijers J, Lansu N, Ruzius FP, Clevers H, Cuppen E

Journal:Nucleic Acids Res

PubMed ID:21914722

'Routine methods for assaying steady-state mRNA levels such as RNA-seq and micro-arrays are commonly used as readouts to study the role of transcription factors (TFs) in gene expression regulation. However, cellular RNA levels do not solely depend on activity of TFs and subsequent transcription by RNA polymerase II (Pol II), ... More

LXRa regulates macrophage arginase 1 through PU.1 and interferon regulatory factor 8.

Authors:Pourcet B, Feig JE, Vengrenyuk Y, Hobbs AJ, Kepka-Lenhart D, Garabedian MJ, Morris SM, Fisher EA, Pineda-Torra I

Journal:Circ Res

PubMed ID:21757649

Activation of liver X receptors (LXRs) inhibits the progression of atherosclerosis and promotes regression of existing lesions. In addition, LXRa levels are high in regressive plaques. Macrophage arginase 1 (Arg1) expression is inversely correlated with atherosclerosis progression and is markedly decreased in foam cells within the lesion. ... More

Resveratrol induces p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated Bax protein oligomerization on mitochondria to initiate cytochrome c release and caspase activation.

Authors:Gogada R, Prabhu V, Amadori M, Scott R, Hashmi S, Chandra D

Journal:J Biol Chem

PubMed ID:21712378

Resveratrol, a naturally occurring phytoalexin, is known to induce apoptosis in multiple cancer cell types, but the underlying molecular mechanisms remain unclear. Here, we show that resveratrol induced p53-independent, X-linked inhibitor of apoptosis protein (XIAP)-mediated translocation of Bax to mitochondria where it underwent oligomerization to initiate apoptosis. Resveratrol treatment promoted ... More