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Thermo Scientific™
Pierce™ Immunodiffusion Plates, Multiple Pattern
Thermo Scientific Pierce Immunodiffusion Plates are standard Ouchterlony gel plates containing patterns of six wells around one well for studyingRead more
| Catalog Number | Quantity |
|---|---|
| 31111 | 6 plates |
Catalog number 31111
Price (CLP)
-
Quantity:
6 plates
Thermo Scientific Pierce Immunodiffusion Plates are standard Ouchterlony gel plates containing patterns of six wells around one well for studying and characterizing antibody-antigen binding interactions.
Features of Pierce Immunodiffusion Plates:
• Gelling agent contains precipitin brighteners for easy visualization
• Immunodiffusion plates contain diffusion enhancers to help speed the interaction process
• Excellent precipitin bands with antibodies from all species (including rabbit antibodies)
• Gels can be washed, dried and stained for a permanent record
Immunodiffusion (ID) is a classic technique for detecting antibody-antigen interactions based on the theory of double diffusion originally described by Oudin and Ouchterlony. Antigens and antibodies are placed into separate wells that are cut into a gel matrix and allowed to diffuse towards each other. If the reaction is positive, a precipitate forms that appears as an opaque line. The precipitation reaction occurs when the antigen and antibody concentrations are combined at near equivalent proportions.
When multivalent antigens combine with divalent antibodies in solution, three-dimensional lattices are formed that aggregate and precipitate. The amount of precipitate varies in proportion to the concentration of the antigen and antibody. At equivalent or optimal proportions almost all the antigen and antibody will precipitate. If there is an excess of antibody, the complexes formed with the antigen are insoluble. When there is an excess of antigen, the precipitate has a tendency to dissolve caused by the formation of soluble complexes.
Features of Pierce Immunodiffusion Plates:
• Gelling agent contains precipitin brighteners for easy visualization
• Immunodiffusion plates contain diffusion enhancers to help speed the interaction process
• Excellent precipitin bands with antibodies from all species (including rabbit antibodies)
• Gels can be washed, dried and stained for a permanent record
Immunodiffusion (ID) is a classic technique for detecting antibody-antigen interactions based on the theory of double diffusion originally described by Oudin and Ouchterlony. Antigens and antibodies are placed into separate wells that are cut into a gel matrix and allowed to diffuse towards each other. If the reaction is positive, a precipitate forms that appears as an opaque line. The precipitation reaction occurs when the antigen and antibody concentrations are combined at near equivalent proportions.
When multivalent antigens combine with divalent antibodies in solution, three-dimensional lattices are formed that aggregate and precipitate. The amount of precipitate varies in proportion to the concentration of the antigen and antibody. At equivalent or optimal proportions almost all the antigen and antibody will precipitate. If there is an excess of antibody, the complexes formed with the antigen are insoluble. When there is an excess of antigen, the precipitate has a tendency to dissolve caused by the formation of soluble complexes.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayImmunodiffusion (ID)
Detection MethodPrecipitate
Product LinePierce
Quantity6 plates
Target SpecificityNot Target-Specific
TechniqueImmunodiffusion (ID)
TypeMulti-Well Plate
Volume (Metric) Working22 μL
ColorClear
Diameter (Metric)3.2 mm
Height (Metric)2.8 mm
Well Count6
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Plates are shipped at ambient temperature. Do not freeze plates or allow them to become dry.