Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Citations & References (1)

Gibco™

Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementationRead more

Catalog Number	Quantity
51985026 also known as 51985-026	500 mL

Catalog number 51985026

also known as 51985-026

Price (CLP)

Quantity:

500 mL

Customize this product

Opti-MEM™ Reduced-Serum Medium is an improved Minimal Essential Medium (MEM) that allows for a reduction of Fetal Bovine Serum supplementation by at least 50% with no change to growth rate or morphology. Opti-MEM™ is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM™ can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts. We offer a variety of Gibco™ Opti-MEM™ modifications for a range of cell culture applications.

This Opti-MEM™ is modified as follows:

• GlutaMAX™

• Phenol Red

The complete formulation is confidential. For more information, please contact Technical Services.

Gibco™ Opti-MEM™ is a unique media that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements. These additional components allow for a reduction in serum supplementation by at least 50%. Gibco™ Opti-MEM™ with GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Gibco™ Opti-MEM™ uses a sodium bicarbonate buffer system (2.4 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Specifications

Cell TypeMammalian Cells

Concentration1 X

Culture EnvironmentCO₂

Manufacturing QualitycGMP-compliant under the ISO 13485 standard

Osmolality280 - 320 mOsm/kg

Product LineGibco, Opti-MEM

Product TypeReduced-Serum Medium

Quantity500 mL

Shelf Life18 Months

Shipping ConditionRoom Temperature

ClassificationAnimal Origin

Culture TypeMammalian Cell Culture

FormLiquid

SterilitySterile-filtered

With AdditivesGlutaMAX, HEPES, Phenol Red, Sodium Pyruvate, Sodium Bicarbonate

pH7 to 7.4

Unit SizeEach

Contents & Storage

Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 18 months from date of manufacture.

Frequently asked questions (FAQs)

Is there any other difference between Opti-MEM I Reduced Serum Medium and Opti-MEM I Reduced Serum Medium, no phenol red besides the presence/absence of phenol red?

The formulation of Opti-MEM I Reduced Serum Medium and that of Opti-MEM I Reduced Serum Medium, no phenol red is the same except for the presence of phenol red in the former medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Where can I find the osmolality for my lot of Opti-MEM I Reduced Serum Medium?

We do provide osmolality information on the certificate of analysis. All lots of Opti-MEM I Reduced Serum Medium will meet the osmolality specification of ≥280 to ≤320 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and freeze Opti-MEM I Reduced Serum Medium to conserve it longer? If so, for how long can it be stored?

Sorry, we don't have data to show that frozen Opti-MEM I Reduced Serum Medium has a longer shelf life. Actually, we recommend storing this medium at 2-8 degrees C in the dark. We do not recommend freezing the medium because it may precipitate.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

I noticed that Opti-MEM I Reduced Serum Medium does not contain any serum. Why do you then refer to it as "Reduced Serum Medium"?

Opti-MEM I Reduced Serum Medium is a serum-free medium. The reason why it is called “reduced-serum medium” is because you only need to supplement this medium with 1-5% FBS instead of the standard 10% FBS.

Opti-MEM I Reduced Serum Medium can be used as is (without serum) for transfection of cells. However, for long-term cell culture, you need to add serum to the medium to keep cells healthy.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Will depletion, absence, or breakdown of essential growth-promoting components such as glutamine or growth factors reduce the growth rate of my culture?

Yes. If you suspect that this is the case, remove the medium and add fresh medium. Alternatively, you can supplement medium with growth-promoting components. It is also possible to substitute GlutaMax I or II for glutamine in the medium to prevent glutamine exhaustion.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Opti-MEM™ I Reduced Serum Medium, GlutaMAX™ Supplement, 500 mL FAQs

Citations & References (1)

Citations & References

Abstract

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.

Authors:Alawar N,Schirra C,Hohmann M,Becherer U

Journal:BMC biotechnology

PubMed ID:38532411

BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and ... More