PrioCHECK™ PRV gE 2.0 Antibody ELISA Kit

The PrioCHECK® PRV gE 2.0 ELISA is a qualitative blocking ELISA for the detection of antibodies in the glycoprotein E (gE) of the Pseudorabies virus (PRV) in pig sera. The samples are incubated in a microtiter plate coated with an inactivated PRV antigen. After washing, the plates are incubated with a monoclonal antibody (mAb) directed against an epitope located on glycoprotein E (gE) of the PRV. This mAb is pre-labeled with an enzyme that generates a color signal. After washing the enzyme substrate is added to the wells of the plates. The signal is measured and when no color is formed, antibodies in the sample have competed for the inactivated PRV antigen. With this result, the sample is positive for PRV.