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Citations & References (4)
Thermo Scientific™
Halt™ Phosphatase Inhibitor Single-Use Cocktail
Thermo Scientific Halt Phosphatase Inhibitor Single Use Cocktail preserves the phosphorylation state of proteins during and after cell lysis orRead more
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| Catalog Number | Quantity |
|---|---|
| 78420 | 1 mL |
| 78428 | 24 x 100 μL |
| 78426 | 5 x 1 mL |
| 78427 | 10 mL |
Catalog number 78420
Price (CLP)
238.614
Each
Quantity:
1 mL
Price (CLP)
238.614
Each
Thermo Scientific Halt Phosphatase Inhibitor Single Use Cocktail preserves the phosphorylation state of proteins during and after cell lysis or tissue protein extraction.
Features of Halt Phosphatase Inhibitor Single Use Cocktail:
• Devoid of protease inhibitors—To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail.
• Dialyzable— Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components.
• Supplied as a 100X concentrate—effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X.
• Compatibility— compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions.
• All-in-one format—inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
• Compatible with popular protein assays—works with standard protein assays, including BCA and coomassie (Bradford) assays
• Convenient storage—refrigerator (2 to 8°C) storage saves valuable space in the freezer and provides for immediate use
Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays.
Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.
Related Products
Pierce™ Phosphatase Inhibitor Mini Tablets
Features of Halt Phosphatase Inhibitor Single Use Cocktail:
• Devoid of protease inhibitors—To protect against both proteases and phosphatases, use Halt Protease and Phosphatase Inhibitor Cocktail.
• Dialyzable— Either dialyze or desalt sample to effectively remove inhibitors from cell extracts before performing procedures that are discovered to be incompatible with cocktail components.
• Supplied as a 100X concentrate—effective when used at a final concentration of 1X; however, samples that contain particularly high levels of phosphatase activity may require a final concentration of 2-3X.
• Compatibility— compatible with most detergent-based cell lysis reagents, including all Thermo Scientific Pierce Cell Lysis Solutions.
• All-in-one format—inhibits both serine-threonine phosphatases and protein tyrosine phosphatases
• Compatible with popular protein assays—works with standard protein assays, including BCA and coomassie (Bradford) assays
• Convenient storage—refrigerator (2 to 8°C) storage saves valuable space in the freezer and provides for immediate use
Halt Phosphatase Inhibitor Cocktail protects phosphoproteins from serine/threonine phosphatases as well as protein tyrosine phosphatases (PTPs) during cell lysis. The cocktail contains a mixture of four inhibitors of broad specificity, including sodium fluoride, sodium orthovanadate, sodium pyrophosphate and beta-glycerophosphate. Conveniently packaged in a 100X format, the cocktail is stable at 4°C and is suitable for use with any cell or tissue extract. The cocktail preserves protein phosphorylation in immunoprecipitation and kinase assays.
Phosphorylation and dephosphorylation comprise a molecular on/off switch that regulates many key biological pathways within the cell, including signal transduction, cell division and apoptosis. To effectively study phosphorylation activation states, one must inactivate phosphatases to prevent their uncontrolled activity upon cell lysis. Halt Phosphatase Inhibitor Cocktail accomplishes this task.
Related Products
Pierce™ Phosphatase Inhibitor Mini Tablets
For Research Use Only. Not for use in diagnostic procedures.
Specifications
InhibitorsPhosphatase Inhibitor
Quantity1 mL
Reagent TypePhosphatase Inhibitor
FormLiquid
Product LineHalt
Product TypePhosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store product at 4°C. Do not freeze.
Frequently asked questions (FAQs)
Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?
Is the Halt Phosphatase Inhibitor Cocktail (Cat. No. 78420) EDTA-free?
I accidentally stored Halt Phosphatase Inhibitor Cocktail Bulk (100 mL) (Cat. No. 78420B) at -20 degrees C. Can I still use it?
Citations & References (4)
Citations & References
Abstract
Metabolic Alterations Caused by Simultaneous Loss of HK2 and PKM2 Leads to Photoreceptor Dysfunction and Degeneration.
Journal:Cells
PubMed ID:37626853
HK2 and PKM2 are two main regulators of aerobic glycolysis. Photoreceptors (PRs) use aerobic glycolysis to produce the biomass necessary for the daily renewal of their outer segments. Previous work has shown that HK2 and PKM2 are important for the normal function and long-term survival of PRs but are dispensable
DYRK2 controls GSTPI expression through ubiquitination and degradation of Twist1 to reduce chemotherapy resistance caused by EMT in breast cancer.
Journal:Journal of molecular histology
PubMed ID:39641870
BACKGROUND: Breast cancer (BC) poses a significant global health challenge, with chemotherapy resistance, especially to docetaxel, remaining a major obstacle in effective treatment. The molecular mechanisms underlying this resistance are critical for developing targeted therapeutic strategies. OBJECTIVE: This study aims to explore the role of dual-specificity tyrosine phosphorylation-regulated kinase 2
Inhibition of Pim kinases triggers a broad antiviral activity by affecting innate immunity and via the PI3K-Akt-mTOR axis the endolysosomal system.
Journal:Antiviral research
PubMed ID:38649071
Zoonoses such as ZIKV and SARS-CoV-2 pose a severe risk to global health. There is urgent need for broad antiviral strategies based on host-targets filling gaps between pathogen emergence and availability of therapeutic or preventive strategies. Significant reduction of pathogen titers decreases spread of infections and thereby ensures health systems
Tyrosine-Based Cross-Linking of Peptide Antigens to Generate Nanoclusters with Enhanced Immunogenicity: Demonstration Using the Conserved M2e Peptide of Influenza A.
Journal:ACS infectious diseases
PubMed ID:34432416
A method of creating nanoclusters (NCs) from soluble peptide molecules is described utilizing an approach based on a tyrosine-tyrosine cross-linking reaction. A reactive tag comprising histidine and tyrosine residues was introduced at the termini of the peptide molecules. The cross-linking reaction led to the creation of dityrosine bonds within the