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Citations & References (6)
Thermo Scientific™
Halt™ Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X)
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) provides the convenience of a single solution with full proteinRead more
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| Catalog Number | Quantity |
|---|---|
| 78445 | 5 x 1 mL |
| 78441 | 1 mL |
| 78443 | 24 x 100 μL |
| 78447 | 10 mL |
Catalog number 78445
Price (CLP)
1.005.708
Each
Quantity:
5 x 1 mL
Price (CLP)
1.005.708
Each
Thermo Scientific Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X) provides the convenience of a single solution with full protein sample protection for cell and tissue lysates.
Features of Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X):
• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers
Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.
Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
Features of Halt Protease and Phosphatase Inhibitor Cocktail, EDTA-free (100X):
• Complete protection—all-in-one cocktail contains both protease and phosphatase inhibitors
• Multiple package sizes—choose from four convenient packages sizes to match nearly any scale
• Convenient —the 100X liquid formulation is much easier and more effective to use than individual inhibitors and other formats
• Easy storage—aqueous-based format allows for convenient cold-room storage without freezing, by contrast with DMSO-based cocktails
• Compatible—can be used in mass spectrometry (MS) because it does not contain AEBSF, which can cause peaks to shift
• Validated—fully compatible with Thermo Scientific Pierce Cell Lysis Buffers
Halt Protease and Phosphatase Inhibitor Cocktail is specifically optimized to protect proteins from degradation during extraction and purification. The cocktail contains inhibitors against the major classes of proteases and phosphatases, targeting aminopeptidases, cysteine and serine proteases and serine/threonine and protein tyrosine phosphatases.
Related Products
Halt™ Protease and Phosphatase Inhibitor Cocktail (100X)
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferLysis Buffer
DescriptionHalt Protease and Phosphatase Inhibitor Cocktail, EDTA-Free
InhibitorsProtease Inhibitor, Phosphatase Inhibitor
Quantity5 x 1 mL
Reagent TypeProtease Inhibitor, Phosphatase Inhibitor
FormLiquid
Product LineHalt
Product TypeProtease and Phosphatase Inhibitor Cocktail
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Product shipped with an ice pack. Do not freeze product.
Frequently asked questions (FAQs)
Can I store the 1X solution of the Halt Protease and Phosphatase Inhbitor Cocktail and if so, for how long?
What are the concentrations of the various components of the combined Halt Protease and Phosphatase Inhibitor Cocktail?
Do you offer the components of the Halt Protease and Phosphatase Cocktail individually?
When do I need to add EDTA when using the Halt Protease and Phosphatase Cocktail?
How much of the Halt Protease and Phosphatase Cocktail do I use for my sample?
Citations & References (6)
Citations & References
Abstract
Impact of carbamazepine on SMARCA4 (BRG1) expression in colorectal cancer: modulation by KRAS mutation status.
Journal:Investigational new drugs
PubMed ID:38446332
SMARCA4 is a gene traditionally considered a tumor suppressor. Recent research has however found that SMARCA4 likely promotes cancer growth and is a good target for cancer treatment. The drug carbamazepine, an autophagy inducer, was used on colorectal cancer cell lines, HCT1116 and Hke3 (KRAS mutant and wildtype). Our study
(3'R)-hydroxytabernaelegantine C: A bisindole alkaloid with potent apoptosis inducing activity in colon (HCT116, SW620) and liver (HepG2) cancer cells.
Journal:Journal of ethnopharmacology
PubMed ID:27616029
ETHNOPHARMACOLOGICAL RELEVANCE: Tabernaemontana elegans Stapf. (Apocynaceae) is a medicinal plant traditionally used in African countries to treat cancer. AIMS OF THE STUDY: To discover new apoptosis inducing lead compounds from T. elegans and provide scientific validation of the ethnopharmacological use of this plant. MATERIALS AND METHODS: Through fractionation, (3'R)-hydroxytaberanelegantine C
Journal:J Nat Prod
PubMed ID:25066282
6-Acetonyldihydrochelerythrine (1), a benzophenanthridine alkaloid, isolated from the methanol extract of Zanthoxylum capense, displayed potent cytotoxic activity in human HCT116 and SW620 colon carcinoma cells, to a higher extent than 5-fluorouracil (5-FU), the cornerstone chemotherapeutic agent in colon cancer. Cytotoxicity of 1 was evaluated by MTS, lactate dehydrogenase (LDH), and
CDK4/6 activity is required during G(2) arrest to prevent stress-induced endoreplication.
Journal:Science (New York, N.Y.)
PubMed ID:38696576
Cell cycle events are coordinated by cyclin-dependent kinases (CDKs) to ensure robust cell division. CDK4/6 and CDK2 regulate the growth 1 (G(1)) to synthesis (S) phase transition of the cell cycle by responding to mitogen signaling, promoting E2F transcription and inhibition of the anaphase-promoting complex. We found that this mechanism
Quantitative PCR analysis of DNA, RNAs, and proteins in the same single cell.
Journal:Clin Chem
PubMed ID:23014600
The single cell represents the basic unit of all organisms. Most investigations have been performed on large cell populations, but understanding cell dynamics and heterogeneity requires single-cell analysis. Current methods for single-cell analysis generally can detect only one class of analytes.