Search

  • Contact Us
  • Quick Order
Fetal Bovine Serum, ultra-low IgG, US origin
Fetal Bovine Serum, ultra-low IgG, US origin
Gibco™

Fetal Bovine Serum, ultra-low IgG, US origin

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantityFormat
A338190150 mLOne Shot
16250078500 mLBottle
Catalog number A3381901
Price (CLP)
175.118
Each
Add to cart
Quantity:
50 mL
Format:
One Shot
Price (CLP)
175.118
Each
Add to cart

Gibco Fetal bovine sera include a broad range of specialty sera, earning the trust of researchers with consistent quality and award-winning support that helps meet your unique research requirements. Ultra-low IgG FBS enhances the quality and accuracy of experimental results by minimizing the potential negative effects of IgG (immunoglobulin G). IgG can interfere with immunological and viral assays or experiments involving antibodies, and some cell types are sensitive to the presence of IgG.

  • Sera category: Specialty
  • Origin: United States
  • Endotoxin level: ≤50 EU/mL (levels routinely ≤10 EU/mL)
  • Hemoglobin level: ≤25 mg/dL

IgG levels are less than 5 μg/mL, and the BVD titer is low or not detectable.

This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.

For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Product LineOne Shot
Shipping ConditionFrozen
SpeciesBovine
AgeFetal
Country of OriginUnited States
FormLiquid
FormatOne Shot
Product TypeFetal Bovine Serum
Quantity50 mL
Serum TreatmentExosome-depleted
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen

Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can serum be stored at 2-8 degrees C?

Serum can be kept at 2-8 degrees C for up to 4 weeks.

Find additional tips, troubleshooting help, and resources within ourMammalian Cell Culture Basics Support Center.

Are there any other packaging configurations available for the new One Shot FBS product?

Yes, we also offer a 10 X 50 mL package, which is equivalent to a 500 mL bottle of FBS.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How is the new One Shot FBS bottle different from our existing One Shot bottle?

Our previous One Shot bottle, a 60 mL Nalgene media bottle, is not designed for easy pipetting and pouring whereas the new One Shot FBS bottle has a propietary design that was developed specifically for FBS use. This design allows you to freeze, thaw and pour without the need to aliquot, which minimizes the amount of FBS handling and reduces the risk of contamination.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does the new One Shot FBS bottle fit into a conical tube rack?

The new One Shot FBS bottle is not shaped to fit into a traditional conical tube rack. It does however fit nicely in a 30 mm test tube rack for the purposes of storage and use in a water bath and biological safety cabinet.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Fetal Bovine Serum, ultra-low IgG, US origin FAQs