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Citations & References (16)
Invitrogen™
Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor™ 488
The Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor™ 488 leverages copper-catalyzed click chemistry and the linoleamide alkyne (LAA) reagentRead more
| Catalog Number | Quantity |
|---|---|
| C10446 | 1 kit |
Catalog number C10446
Price (CLP)
753.708
Each
Quantity:
1 kit
Price (CLP)
753.708
Each
The Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor™ 488 leverages copper-catalyzed click chemistry and the linoleamide alkyne (LAA) reagent (alkyne-modified linoleic acid) for detection of lipid peroxidation-derived protein modifications in fixed cells. Linoleic acid is the most abundant polyunsaturated fatty acid found in mammals and its lipid peroxidation products likely account for the majority of lipid-derived protein carbonyls.
The Click-iT™ Lipid Peroxidation Imaging Kit is:
• Optimized for detection of lipid peroxidation in cells using click chemistry and Alexa Fluor™ 488 azide
• Multiplexible and compatible with traditional fluorescence microscopy, high content screening (HCS), and flow cytometry
• Provided as a complete kit sufficient for five 96-well plates or 100 coverslips
How It Works
The Click-iT™ Lipid Peroxidation Imaging Kit contains cumene hydroperoxide as a positive control for induction of lipid peroxidation and all of the components for the click reaction, including LAA, and Alexa Fluor™ 488 azide. LAA can be incubated with cells, where it incorporates into cellular membranes. Upon lipid peroxidation, LAA is oxidized and produces 9- and 13- hydroperoxy-octadecadienoic acid (HPODE). These hydroperoxides decompose to multiple α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains. These alkyne-containing modified proteins can be subsequently detected using Click-iT™ chemistry and multiplexed with other probes appropriate for fixed cells. This assay is amenable to detection by traditional fluorescence microscopy, high content screening (HCS), and flow cytometry.
Available in Two Formats
The Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor ™ 488 contains the components you need to detect lipid peroxidation in cells, including positive control compound. Alternatively, Click-iT™ LAA (linoleamide alkyne) is a stand-alone reagent provided as a five pack set of single-use vials for maximum flexibility in assays for lipid peroxidation. This reagent may be combined with a variety of azide-modified detection reagents and other reagents in the Click-iT™ tool box.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
The Click-iT™ Lipid Peroxidation Imaging Kit is:
• Optimized for detection of lipid peroxidation in cells using click chemistry and Alexa Fluor™ 488 azide
• Multiplexible and compatible with traditional fluorescence microscopy, high content screening (HCS), and flow cytometry
• Provided as a complete kit sufficient for five 96-well plates or 100 coverslips
How It Works
The Click-iT™ Lipid Peroxidation Imaging Kit contains cumene hydroperoxide as a positive control for induction of lipid peroxidation and all of the components for the click reaction, including LAA, and Alexa Fluor™ 488 azide. LAA can be incubated with cells, where it incorporates into cellular membranes. Upon lipid peroxidation, LAA is oxidized and produces 9- and 13- hydroperoxy-octadecadienoic acid (HPODE). These hydroperoxides decompose to multiple α,β-unsaturated aldehydes, which readily modify proteins at nucleophilic side chains. These alkyne-containing modified proteins can be subsequently detected using Click-iT™ chemistry and multiplexed with other probes appropriate for fixed cells. This assay is amenable to detection by traditional fluorescence microscopy, high content screening (HCS), and flow cytometry.
Available in Two Formats
The Click-iT™ Lipid Peroxidation Imaging Kit - Alexa Fluor ™ 488 contains the components you need to detect lipid peroxidation in cells, including positive control compound. Alternatively, Click-iT™ LAA (linoleamide alkyne) is a stand-alone reagent provided as a five pack set of single-use vials for maximum flexibility in assays for lipid peroxidation. This reagent may be combined with a variety of azide-modified detection reagents and other reagents in the Click-iT™ tool box.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorRed, Green
FormatFrozen
Quantity1 kit
Unit SizeEach
Citations & References (16)
Citations & References
Abstract
Immuno-spin trapping of heme-induced protein radicals: Implications for heme oxygenase-1 induction and heme degradation.
Journal:Free Radic Biol Med
PubMed ID:23624303
'Heme, in the presence of hydrogen peroxide, can act as a peroxidase. Intravascular hemolysis results in a massive release of heme into the plasma in several pathophysiological conditions such as hemolytic anemia, malaria, and sickle cell disease. Heme is known to induce heme oxygenase-1(HO-1) expression, and the extent of induction
Inflammasome activation in airway epithelial cells after multi-walled carbon nanotube exposure mediates a profibrotic response in lung fibroblasts.
Journal:
PubMed ID:24915862
In vivo studies have demonstrated the ability of multi-walled carbon nanotubes (MWCNT) to induce airway remodeling, a key feature of chronic respiratory diseases like asthma and chronic obstructive pulmonary disease. However, the mechanism leading to remodeling is poorly understood. Particularly, there is limited insight about the role of airway epithelial
Hemoglobin induced lung vascular oxidation, inflammation, and remodeling contributes to the progression of hypoxic pulmonary hypertension and is attenuated in rats with repeat dose haptoglobin administration.
Journal:
PubMed ID:25656991
Haptoglobin (Hp) is an approved treatment in Japan with indications for trauma, burns and massive transfusion related hemolysis. Additional case reports suggest uses in other acute hemolytic events that lead to acute kidney injury. However, Hp's protective effects on the pulmonary vasculature have not been evaluated within the context of
Mitochondrial membrane potential in 2-cell stage embryos correlates with the success of preimplantation development.
Journal:
PubMed ID:24459207
Hormonal stimulation in superovulation induces female mice to ovulate more oocytes than spontaneous ovulation. Because the superovulated oocytes contain a number of oocytes that normally regress before spontaneous ovulation or immature oocytes, the development of some embryos that derive from these oocytes by IVF is prevented. Therefore, the quality of
De-differentiation confers multidrug resistance via noncanonical PERK-Nrf2 signaling.
Journal:
PubMed ID:25203443
Malignant carcinomas that recur following therapy are typically de-differentiated and multidrug resistant (MDR). De-differentiated cancer cells acquire MDR by up-regulating reactive oxygen species (ROS)-scavenging enzymes and drug efflux pumps, but how these genes are up-regulated in response to de-differentiation is not known. Here, we examine this question by using global