5-(and-6)-Carboxy SNARF™-1
5-(and-6)-Carboxy SNARF™-1
Citations & References (43)
Invitrogen™

5-(and-6)-Carboxy SNARF™-1

The cell-impermeant pH indicator, carboxy SNARF™-1 dye has a pKa of ∼7.5, thus is useful for measuring pH changes betweenRead more
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Catalog NumberQuantity
C12701 mg
Catalog number C1270
Price (CLP)
549.817
Each
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Quantity:
1 mg
Price (CLP)
549.817
Each
Add to cart
The cell-impermeant pH indicator, carboxy SNARF™-1 dye has a pKa of ∼7.5, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF™-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths - typically 580 nm and 640 nm - to be used for quantitative determinations of pH.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypepH Indicator
Quantity1 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer, Microplate Reader
Product LineSNARF
Product TypepH Indicator
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.

Citations & References (43)

Citations & References
Abstract
Simultaneous measurement of intracellular pH and Ca2+ in insulin-secreting cells by spectral imaging microscopy.
Authors:Martínez-Zaguilán R,Gurulé MW,Lynch RM
Journal:The American journal of physiology
PubMed ID:8967445
Measurement of intracellular pH.
Authors:Boyer MJ, Hedley DW
Journal:Methods Cell Biol
PubMed ID:7532259
Fluorescent indicators of ion concentrations.
Authors:Tsien RY
Journal:Methods Cell Biol
PubMed ID:2538708
Comparison of spectrum-shifting intracellular pH probes 5'(and 6')-carboxy-10-dimethylamino-3-hydroxyspiro[7H-benzo[c]xanthene-7, 1'(3'H)-isobenzofuran]-3'-one and 2',7'-biscarboxyethyl-5(and 6)-carboxyfluorescein.
Authors:Owen CS
Journal:Anal Biochem
PubMed ID:1514696
'The dyes carboxy-SNARF-1 and BCECF are fluorescent probes of intracellular pH that exhibit changes in spectral shape upon proton binding which allow one to use measurements of fluorescence at two or more wavelengths in order to measure pH without artifacts associated with variability in dye loading, etc. In evaluating these ... More
Profiling pH changes in the electrospray plume.
Authors:Zhou S, Prebyl BS, Cook KD
Journal:Anal Chem
PubMed ID:12380808
'Laser-induced fluorescence spectrometry is used to profile pH changes as droplets evaporate in an electrospray plume by measuring emission spectra of 2(or 4)-[10-(dimethylamino)-3-oxo-3H-benzo[c]xanthene-7-yl]-benzenedicarboxylic acid (carboxy SNARF-1), a pH-sensitive fluorescent dye. The observed pH changes depend on initial droplet pH and polarity. In some instances, small or negligible changes of pH ... More
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