LC3B Antibody Kit for Autophagy
LC3B Antibody Kit for Autophagy
Citations & References (19)
Invitrogen™

LC3B Antibody Kit for Autophagy

The LC3 Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use inRead more
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Catalog NumberQuantity
L103821 kit
Catalog number L10382
Price (CLP)
611.672
Each
Add to cart
Quantity:
1 kit
Price (CLP)
611.672
Each
Add to cart
The LC3 Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. Chloroquine diphosphate is also included - following treatment with this compound, normal autophagic flux is disrupted and autophagosomes accumulate as a result of the disruption in normal autophagic flux.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeUnlabeled
Format96-well plate, Slide(s)
Quantity1 kit
Shipping ConditionDry Ice
For Use With (Equipment)Confocal Microscope, Fluorescence Microscope, High Content Analysis Instrument
Product TypeDye
Unit SizeEach
Contents & Storage
Kit includes includes a rabbit polyclonal antibody against LC3B and chloroquine diphosphate.
  • Store at ≤ -20°C

    • Frequently asked questions (FAQs)

    How does the LC3B Antibody Kit for Autophagy work?

    The LC3B protein plays a critical role in autophagy. This protein resides in the cytosol, but following cleavage and lipidation with phosphatidylethanolamine, LC3 associates with the phagophore. This localization can be used as a general marker for autophagic membranes. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high content imaging and analysis. The kit also includes chloroquine diphosphate for artificially generating autophagosomes. Following chloroquine diphosphate treatment, lysosomal pH increases and the normal autophagic flux is disrupted, resulting in autophagosome accumulation.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Citations & References (19)

    Citations & References
    Abstract
    WIPI1 coordinates melanogenic gene transcription and melanosome formation via TORC1 inhibition.
    Authors:Ho H, Kapadia R, Al-Tahan S, Ahmad S, Ganesan AK,
    Journal:J Biol Chem
    PubMed ID:21317285
    'Recent studies implicate a role for WD repeat domain, phosphoinositide-interacting 1 (WIPI1) in the biogenesis of melanosomes, cell type-specific lysosome-related organelles. In this study, we determined that WIPI1, an ATG18 homologue that is shown to localize to both autophagosomes and early endosomes, inhibited mammalian target of rapamycin (MTOR) signaling, leading ... More
    Membrane fusion-mediated autophagy induction enhances morbillivirus cell-to-cell spread.
    Authors:Delpeut S, Rudd PA, Labonté P, von Messling V,
    Journal:J Virol
    PubMed ID:22647692
    In the context of viral infections, autophagy induction can be beneficial or inhibitory. Within the Paramyxoviridae family, only morbilliviruses have been investigated and are reported to induce autophagy. Here we show that morbilliviruses rapidly induce autophagy and require this induction for efficient cell-to-cell spread. Coexpression of both glycoproteins in cells ... More
    Hormonally active vitamin D3 (1alpha,25-dihydroxycholecalciferol) triggers autophagy in human macrophages that inhibits HIV-1 infection.
    Authors:Campbell GR, Spector SA,
    Journal:J Biol Chem
    PubMed ID:21454634
    Autophagy is a self-digestion pathway essential for maintaining cellular homeostasis and cell survival and for degrading intracellular pathogens. Human immunodeficiency virus-1 (HIV-1) may utilize autophagy for replication as the autophagy-related protein-7 (ATG-7), microtubule-associated protein 1 light chain 3, ATG-12, and ATG-16L2 are required for productive HIV-1 infection; however, the effects ... More
    Galectin-8 Senses Phagosomal Damage and Recruits Selective Autophagy Adapter TAX1BP1 To Control Mycobacterium tuberculosis Infection in Macrophages.
    Authors:
    Journal:MBio
    PubMed ID:34225486
    Malpighian Tubule Cells in Overwintering Cave Crickets Troglophilus cavicola (Kollar, 1833) and T. neglectus Krauss, 1879 (Rhaphidophoridae, Ensifera).
    Authors:Lipovšek S, Novak T, Janžekovic F, Weiland N, Leitinger G
    Journal:PLoS One
    PubMed ID:27379687
    'During winter, cave cricket larvae undergo dormancy in subterranean habitats; this dormancy is termed diapause in second year Troglophilus cavicola larvae because they mature during this time, and termed quiescence in T. neglectus, because they mature after dormancy. Here we used electron microscopy to analyze ultrastructural changes in the epithelial ... More
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