2-NBDG is a fluorescent glucose analog that has been used to monitor glucose uptake in live cells, as an indicator of cell viability. Although sensitive to its environment NBD fluorescence typically displays excitation/emission maxima of ∼465/540 nm and can be visualized using optical filters designed for fluorescein.

Dissolve the dye in water or 1X PBS (pH 7.2) to prepare a stock solution up to 10 mg/mL. Stock solutions should be prepared fresh and used on same day.