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Citations & References (54)
Invitrogen™
Quant-it™ RiboGreen Reagent and RNA Assay Kit
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
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| Catalog Number | Quantity | Product Type | Quantitation Range |
|---|---|---|---|
| R11490 | 1 kit | Quant-iT RiboGreen RNA Kit | 1 to 200 ng |
| R11491 | 1 mL | Quant-iT RiboGreen RNA Reagent | 1 to 200 ng |
| R32700 | 1 kit | RediPlate 96 RiboGreen RNA Kit | 3 to 200 ng |
Catalog number R11490
Price (CLP)
734.654
Each
Quantity:
1 kit
Product Type:
Quant-iT RiboGreen RNA Kit
Quantitation Range:
1 to 200 ng
Price (CLP)
734.654
Each
Quant-iT RiboGreen RNA Reagent is an ultrasensitive fluorescent nucleic acid stain for quantitating RNA in solution. The Quant-iT RiboGreen RNA Assay Kit comes with reagent, dilution buffer, and rRNA standards to provide a linear detection range of 1–200 ng of RNA.
Workflow
This easy-to-use reagent offers two detection ranges: a high-assay range and a low-assay range for sensitive and broad range detection. To use, simply prepare the working solution by diluting the provided dye with TE-Buffer, then aliquotting 100 µL of the working solution with standards and samples for a total reaction volume of 200 µL. RNA is then quantitated using a fluorescence microplate reader (such as Varioskan ALF) at standard fluorescein wavelengths.
Applications
Detecting and quantitating small amounts of RNA is important in many applications including measuring yields of in vitro transcribed RNA and measuring RNA concentrations before performing Northern blot analyses, S1 nuclease assays, RNase protection assays, cDNA library preparation, reverse transcription PCR, and differential display PCR.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission500/525
For Use With (Equipment)Microplate Reader
No. of Reactions200-2,000 cuvette assays using a 2 mL assay volume
Product LineQuant-iT, RIBOGREEN
Product TypeQuant-iT RiboGreen RNA Kit
Quantitation Range1 to 200 ng
Quantity1 kit
Shipping ConditionRoom Temperature
Detection MethodFluorescence
Unit SizeEach
Frequently asked questions (FAQs)
Why am I getting negative fluorescence values with my Qubit Assays?
What are the linear ranges of the Quant-iT RNA kits?
What is the difference between the Quant-iT RNA assay and the Quant-iT RiboGreen RNA Assay?
Can I make my own assay for the Qubit Fluorometer?
I have a crude lysate. Will the Quant-iT and Qubit assays work?
Citations & References (54)
Citations & References
Abstract
microRNAs exhibit high frequency genomic alterations in human cancer.
Journal:Proceedings of the National Academy of Sciences of the United States of America
PubMed ID:16754881
MicroRNAs (miRNAs) are endogenous noncoding RNAs, which negatively regulate gene expression. To determine genomewide miRNA DNA copy number abnormalities in cancer, 283 known human miRNA genes were analyzed by high-resolution array-based comparative genomic hybridization in 227 human ovarian cancer, breast cancer, and melanoma specimens. A high proportion of genomic loci
A fluorescence-based assay for multisubunit DNA-dependent RNA polymerases.
Journal:Anal Biochem
PubMed ID:14690681
The properties of DNA-dependent RNA polymerases have been studied since the 1960s, but considerable interest in probing RNA polymerase structure/function relationships, the roles of different classes of RNA polymerases in cellular processes, and the feasibility of using RNA polymerases as drug targets still exists. Historically, RNA polymerase activity has been
Quantification of mRNA using real-time RT-PCR.
Journal:Nat Protoc
PubMed ID:17406449
'The real-time reverse transcription polymerase chain reaction (RT-qPCR) addresses the evident requirement for quantitative data analysis in molecular medicine, biotechnology, microbiology and diagnostics and has become the method of choice for the quantification of mRNA. Although it is often described as a "gold" standard, it is far from being a
A systemic small RNA signaling system in plants.
Journal:Plant Cell
PubMed ID:15258266
'Systemic translocation of RNA exerts non-cell-autonomous control over plant development and defense. Long-distance delivery of mRNA has been proven, but transport of small interfering RNA and microRNA remains to be demonstrated. Analyses performed on phloem sap collected from a range of plants identified populations of small RNA species. The dynamic
Fibrinogen induces IL-8 synthesis in human neutrophils stimulated with formyl-methionyl-leucyl-phenylalanine or leukotriene B(4).
Journal:J Immunol
PubMed ID:11509634
'Human exudative neutrophils have greatly increased stores of the neutrophil chemoattractant IL-8 compared with peripheral blood cells, but the mechanism for the increase is not defined. In this report, we show that treatment of peripheral blood neutrophils with the chemotactic peptide fMLP or with leukotriene B(4) or fibrinogen results in