Zeocin™ Selection Reagent
Zeocin™ Selection Reagent
Citations & References (66)
Gibco™

Zeocin™ Selection Reagent

Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus.
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Catalog NumberQuantity
R250018 x 1.25 mL
R2500550 mL
Catalog number R25001
Price (CLP)
348.515
Each
Add to cart
Quantity:
8 x 1.25 mL
Price (CLP)
348.515
Each
Add to cart

Gibco Zeocin Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus. It acts by binding to and cleaving DNA, leading to cell death, and is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures. Working concentration range is 50–400 μg/mL depending on the cell type. This product is supplied at 100 mg/mL in sterile water in 8 x 1.25 mL vials.

  • Gibco™ Zeocin™ Selection Antibiotic is a formulation of phleomycin D1, a glycopeptide antibiotic produced by Streptomyces verticillus
  • Zeocin™ Selection Antibiotic acts by binding to and cleaving DNA, leading to cell death
  • It is used for selection of of mammalian, insect, yeast, bacterial, and plant cell cultures
  • Working concentration ranges between 50–400 μg/mL depending on the cell type
  • This product is supplied at 100 mg/mL in sterile water, in 8 x 1.25 mL vials
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 mg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
For Use With (Application)Eukaryotic Selection/Stable Cell Line Generation, Bacterial Selection
Product LineZeocin
Quantity8 x 1.25 mL
FormLiquid
Product TypeAntibiotic
SterilitySterile-filtered
Unit SizeEach
Contents & Storage
Storage conditions: -10 to -30°C
Shipping conditions: Ice

Frequently asked questions (FAQs)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

I received Zeocin and it looks blue. Is this normal?

Zeocin is a formulation of phleomycin D1, a basic, water-soluble, copper-chelated glycopeptide isolated from Streptomyces verticillus. The presence of copper gives the solution its blue color. This copper-chelated form is inactive. When the antibiotic enters the cell, the copper cation is reduced from Cu2+ to Cu1+ and removed by sulfhydryl compounds in the cell. Upon removal of the copper, Zeocin is activated and will bind DNA and cleave it, causing cell death.

What is the molecular weight of Zeocin?

MW=1,535. Molecular formula: C60H89N21O21S3.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What safety precautions should be taken when autoclaving Zeocin-containing media and plates before disposal? Are there any long-term effects for people who are exposed to Zeocin on a daily basis?

Zeocin, in media or other liquid form, is a health hazard when it comes in contact with your skin or upon ingestion. When it is in the powder solid form, it can become a respiratory hazard as well as contact and ingestion hazard. If the liquid or media is placed in an autoclavable bag, it can be autoclaved. The elevated temperatures within the autoclave will inactivate Zeocin. Sodium hypochloride (bleach) will also inactivate Zeocin. We highly recommend wearing the appropriate personal protective equipment (lab coat, gloves, and eye protection) when handling Zeocin.

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Citations & References (66)

Citations & References
Abstract
DBC2, a candidate for a tumor suppressor gene involved in breast cancer.
Authors:Hamaguchi Masaaki; Meth Jennifer L; von Klitzing Christine; Wei Wen; Esposito Diane; Rodgers Linda; Walsh Tom; Welcsh Piri; King Mary-Claire; Wigler Michael H;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12370419
A previously uncharacterized gene, DBC2 (deleted in breast cancer), was cloned from a homozygously deleted region at human chromosome 8p21. DBC2 contains a highly conserved RAS domain and two putative protein interacting domains. Our analyses indicate that DBC2 is the best candidate tumor suppressor gene from this region. It lies ... More
Neuropilin-1 binds vascular endothelial growth factor 165, placenta growth factor-2, and heparin via its b1b2 domain.
Authors: Mamluk Roni; Gechtman Ze'ev; Kutcher Matthew E; Gasiunas Nijole; Gallagher John; Klagsbrun Michael;
Journal:J Biol Chem
PubMed ID:11986311
'Neuroplin-1 (NRP1), a receptor for vascular endothelial growth factor (VEGF) family members, has three distinct extracellular domains, a1a2, b1b2, and c. To determine the VEGF(165) and placenta growth factor 2 (PlGF-2)-binding sites of NRP1, recombinant NRP1 domains were expressed in mammalian cells as Myc-tagged, soluble proteins, and used in co-precipitation ... More
TID1, a human homolog of the Drosophila tumor suppressor l(2)tid, encodes two mitochondrial modulators of apoptosis with opposing functions.
Authors:Syken J, De-Medina T, Munger K
Journal:Proc Natl Acad Sci U S A
PubMed ID:10411904
'Mitochondria have emerged as central regulators of apoptosis. Here, we show that TID1, a human homolog of the Drosophila tumor suppressor lethal (2) tumorous imaginal discs, l(2)tid, encodes two mitochondrial matrix proteins, designated hTid-1(L) and hTid-1(S). These splice variants are both highly conserved members of the DnaJ family of proteins, ... More
Human mitochondrial 5'-deoxyribonucleotidase. Overproduction in cultured cells and functional aspects.
Authors: Gallinaro Lisa; Crovatto Katia; Rampazzo Chiara; Pontarin Giovanna; Ferraro Paola; Milanesi Eva; Reichard Peter; Bianchi Vera;
Journal:J Biol Chem
PubMed ID:12124385
'Deoxynucleoside triphosphates (dNTPs) used for mitochondrial DNA replication are mainly formed by phosphorylation of deoxynucleosides imported into mitochondria from the cytosol. We earlier obtained evidence for a mitochondrial 5''-nucleotidase (dNT2) with a pronounced specificity for dUMP and dTMP and suggested that the enzyme protects mitochondrial DNA replication from excess dTTP. ... More
Cellular response to oncogenic ras involves induction of the Cdk4 and Cdk6 inhibitor p15(INK4b).
Authors:Malumbres M, Perez De Castro I, Hernandez MI, Jimenez M, Corral T, Pellicer A
Journal:Mol Cell Biol
PubMed ID:10733595
'The cell cycle inhibitor p15(INK4b) is frequently inactivated by homozygous deletion together with p16(INK4a) and p19(ARF) in some types of tumors. Although the tumor suppressor capability of p15(INK4b) is still questioned, it has been found to be specifically inactivated by hypermethylation in hematopoietic malignancies in the absence of p16(INK4a) alterations. ... More
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