ADN polimerasa Taq, recombinante
ADN polimerasa <i>Taq</i>, recombinante
ADN polimerasa <i>Taq</i>, recombinante
ADN polimerasa <i>Taq</i>, recombinante
Citas y referencias (4)
Invitrogen™

ADN polimerasa Taq, recombinante

La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de plantillas monocatenarias en presencia deMás información
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Número de catálogoN.º de reacciones
10342020500 reacciones
10342053100 reacciones
103420461500 reacciones
103421785000 reacciones
Número de catálogo 10342020
Precio (CLP)
330.693
Each
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N.º de reacciones:
500 reacciones
Pedido a granel o personalizado
Precio (CLP)
330.693
Each
Añadir al carro de la compra
La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de plantillas monocatenarias en presencia de dNTP y un primer. La enzima se compone de un polipéptido único con un peso molecular de 94 kDa. Tiene una actividad de ADN polimerasa de 5´→3´ y una actividad de exonucleasa de 5´→3´.

Con nuestra ADN polimerasa Taq podrá:

• Posibilidad de seleccionar la enzima nativa o recombinante
• Amplificación de productos de PCR con un tamaño máximo de 5 kb
• Una enzima que posee licencia y está certificada para PCR

Aplicaciones
La ADN polimerasaTaq es adecuada para su uso en la amplificación de ADN a partir de complejos moldes genómicos, virales y plasmídicos, RT-PCR, ADNss de secuenciación y secuenciación de ciclos.

Fuente
La enzima recombinante se purifica mediante el gen de ADN polimerasa Thermus aquaticus clonado expresado en E. coli.

Definición de unidad
Una unidad de ADN polimerasa Taq es la cantidad de enzima necesaria para incorporar 10 nmoles de desoxirribonucleótido en ADN en 30 min a 74 °C.

Para un rendimiento superior de PCR, recomendamos ADN polimerasa DreamTaq.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Actividad exonucleasa5' - 3'
Fidelidad (frente a Taq)1 X
FormatoEnzima independiente
Inicio en calienteNo
N.º de reacciones500 reacciones
Sobrante3'-A
PolimerasaADN polimerasa Taq
Tipo de productoADN polimerasa
Cantidad500 unidades
Formato de reacciónComponentes separados
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tamaño (producto final)5 kb o menos
Material de partidaADN
Concentración5 U/μl
Para utilizar con (aplicación)Standard PCR
GC-Rich PCR PerformanceBajo
Método de PCRqPCR, PCR estándar
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
Contiene:
• 100 μl de ADN polimerasa Taq (5 U/μl)
• Tampón para PCR 10X de 2,5 ml (200 mm de Tris-HCl, pH de 8,4 y 500 mm de KCl)
• 1 ml de cloruro magnésico (50 mm)

Almacenar a una temperatura de entre -30 °C y -10 °C en un congelador que no forme escarcha. Estabilidad garantizada durante seis meses si se almacena correctamente.

Preguntas frecuentes

My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

I don't see a pellet in my oligo tube order. Should I ask for a replacement?

The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

There is a green color in my lyophilized oligo. Can I still use it?

If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

View all ADN polimerasa Taq, recombinante FAQs

Citations & References (4)

Citations & References
Abstract
alpha-Methylacyl coenzyme A racemase as a tissue biomarker for prostate cancer.
Authors: Rubin Mark A; Zhou Ming; Dhanasekaran Saravana M; Varambally Sooryanarayana; Barrette Terrence R; Sanda Martin G; Pienta Kenneth J; Ghosh Debashis; Chinnaiyan Arul M;
Journal:JAMA
PubMed ID:11926890
'Edited due to space constraints: CONTEXT: Molecular profiling of prostate cancer has led to the identification of candidate biomarkers and regulatory genes. Discoveries from these genome-scale approaches may have applicability in the analysis of diagnostic prostate specimens. OBJECTIVES: To determine the expression and clinical utility of alpha-methylacyl coenzyme A racemase ... More
Novel isoforms of the sodium channels Nav1.8 and Nav1.5 are produced by a conserved mechanism in mouse and rat.
Authors:Kerr NC, Holmes FE, Wynick D,
Journal:J Biol Chem
PubMed ID:15047701
The voltage-gated sodium channel Nav1.8 is only expressed in subsets of neurons in dorsal root ganglia (DRG), trigeminal and nodose ganglia. We have isolated mouse partial-length Nav1.8 cDNA clones spanning exon 17 sequence which have 17 nucleotide substitutions and 12 predicted amino acid differences from the published sequence. The absence ... More
Low density lipoprotein receptor-related protein mediates apolipoprotein E inhibition of smooth muscle cell migration.
Authors: Swertfeger Debi K; Bu Guojun; Hui David Y;
Journal:J Biol Chem
PubMed ID:11739389
This research was undertaken to identify the cell surface receptor responsible for mediating apolipoprotein E (apoE) inhibition of platelet-derived growth factor (PDGF)-directed smooth muscle cell migration. Initial studies revealed the expression of the low density lipoprotein receptor (LDLR), the LDL receptor-related protein (LRP), the very low density lipoprotein receptor (VLDL), ... More
Association between CYP2C9 genetic variants and anticoagulation-related outcomes during warfarin therapy.
Authors: Higashi Mitchell K; Veenstra David L; Kondo L Midori; Wittkowsky Ann K; Srinouanprachanh Sengkeo L; Farin Fred M; Rettie Allan E;
Journal:JAMA
PubMed ID:11926893
Edited for space constraints: Warfarin is a commonly used anticoagulant that requires careful clinical management to balance the risks of overanticoagulation and bleeding with those of underanticoagulation and clotting. The principal enzyme involved in warfarin metabolism is CYP2C9, and 2 relatively common variant forms with reduced activity have been identified, ... More