PFASTBAC 1 EXPRESSION VECTOR

Name: PFASTBAC 1 EXPRESSION VECTOR
SKU: 10360010

Número de catálogo 10360010

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Citations & References (6)

Citations & References

Abstract

Baculoviral vector-mediated transient and stable transgene expression in human embryonic stem cells.

Authors:Zeng J, Du J, Zhao Y, Palanisamy N, Wang S

Journal:Stem Cells

PubMed ID:17420229

'Human embryonic stem (hES) cells as a renewable cell source have great prospective applications in both developmental biology research and regenerative medicine. To realize these potentials, the development of effective and safe genetic manipulation methods in hES cells is an obvious demand. We report here that baculoviral vectors were able ... More

Cloning and characterization of PDE7B, a cAMP-specific phosphodiesterase.

Authors:Hetman JM, Soderling SH, Glavas NA, Beavo JA

Journal:Proc Natl Acad Sci U S A

PubMed ID:10618442

A member of the phosphodiesterase (PDE)7 family with high affinity and specificity for cAMP has been identified. Based on sequence homologies, we designate this PDE as PDE7B. The full-length cDNA of PDE7B is 2399 bp, and its ORF sequence predicts a protein of 446 amino acids with a molecular mass ... More

The orexin OX1 receptor regulates Ca2+ entry via diacylglycerol-activated channels in differentiated neuroblastoma cells.

Authors:Näsman J, Bart G, Larsson K, Louhivuori L, Peltonen H, Akerman KE,

Journal:J Neurosci

PubMed ID:17050705

We studied the cellular response to orexin type 1 receptor (OX1R) stimulation in differentiated IMR-32 neuroblastoma cells. In vitro differentiation of IMR-32 cells with 5-bromo-2'-deoxyuridine leads to a neuronal phenotype with long neurite extensions and an upregulation of mainly N-type voltage-gated calcium channels. Transduction of differentiated IMR-32 cells with baculovirus ... More

RAD51C Interacts with RAD51B and Is Central to a Larger Protein Complex in Vivo Exclusive of RAD51.

Authors: Miller Kristi A; Yoshikawa Daniel M; McConnell Ian R; Clark Robin; Schild David; Albala Joanna S;

Journal:J Biol Chem

PubMed ID:11744692

RAD51B and RAD51C are two of five known paralogs of the human RAD51 protein that are thought to function in both homologous recombination and DNA double-strand break repair. This work describes the in vitro and in vivo identification of the RAD51B/RAD51C heterocomplex. The RAD51B/RAD51C heterocomplex was isolated and purified by ... More

Gene characterized for membrane desaturase that produces (E)-11 isomers of mono- and diunsaturated fatty acids.

Authors: Liu Weitian; Jiao Hongmei; Murray Nancy C; O'Connor Marion; Roelofs Wendell L;

Journal:Proc Natl Acad Sci U S A

PubMed ID:11805319

Moth species have evolved integral membrane desaturases that exhibit a wide diversity in substrate specificity, as well as in regiospecificity and stereospecificity of the unsaturated products. We report here the cloning and expression of a single desaturase from the sex pheromone gland of the light brown apple moth, Epiphyas postvittana, ... More

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