Embryonic stem-cell FBS, qualified, USDA-approved regions
Embryonic stem-cell FBS, qualified, USDA-approved regions
Gibco™

Embryonic stem-cell FBS, qualified, USDA-approved regions

Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayosMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidadFormato
10439024500 mLFrasco
1043900150 mLOne Shot
Número de catálogo 10439024
Precio (CLP)
1.053.816
Each
Añadir al carro de la compra
Cantidad:
500 mL
Formato:
Frasco
Precio (CLP)
1.053.816
Each
Añadir al carro de la compra
Los sueros bovinos fetales Gibco™ ofrecen un excelente valor para el cultivo celular básico, la investigación especializada y los ensayos específicos, ganando la confianza de los investigadores con una calidad uniforme y un soporte galardonado que ayuda a satisfacer sus necesidades de investigación y presupuesto

Categoría de los sueros: Especialidad
Origen: Países que cumplen los requisitos de importación del USDA.
Se han sometido a pruebas específicas para comprobar la capacidad de mantener la morfología celular no diferenciada de las células madre embrionarias.
Nivel de endotoxinas: ≤50 EU/ml (niveles habituales ≤10 EU/ml).
Nivel de hemoglobina: ≤25 mg/dl.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Concentración de endotoxinas<50 EU/mL
Concentración de hemoglobina≤25 mg/dL
Grado de pureza o calidadVálido para células madre embrionarias
Condiciones de envíoCongelado
EspecieGanado/bovino
AgeFetal
País de origenMéxico
FormularioLíquido
FormatoFrasco
Tipo de productoSuero fetal bovino
Cantidad500 mL
Serum TreatmentEstándar (esterilizado mediante filtro)
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Embryonic stem-cell FBS, qualified, USDA-approved regions, 500 mL FAQs