MaV203 Competent Yeast Cells, Subcloning Scale

Las células de levadura competentes MaV203 están diseñadas para su uso con el sistema de dos híbridos ProQuest™ (figura 1).Más información

Número de catálogo	Cantidad
11445012	4 x 0,1 mL

Número de catálogo 11445012

Precio (CLP)

788.071

Añadir al carro de la compra

Cantidad:

4 x 0,1 mL

Precio (CLP)

788.071

Añadir al carro de la compra

Las células de levadura competentes MaV203 están diseñadas para su uso con el sistema de dos híbridos ProQuest™ (figura 1). La cepa S. cerevisiae MaV203 contiene eliminaciones en genes GAL4y GAL80 endógenos para su uso con sistemas de dos híbridos basados en GAL4. La cepa también tiene mutaciones leu2 y trp1 para la selección de vectores de cebo y presa ProQuest™. MaV203 contiene tres genes reporteros inducibles GAL4, que proporcionan cuatro fenotipos, para facilitar la identificación de los verdaderos interactores (Figura 2). Las células competentes MaV203 incluyen:

• Producciones de eficacia de transformación (250 µ l de células) colonias de >1 x 10⁶
• Controlar el ADN pMAB12 para confirmar la eficacia de la transformación y la preparación de medios de selección
• PEG/acetato de litio precalificados para maximizar el número de transformantes
• Células competentes con un formato de ADN portador ya incluido.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Cepa bacteriana o de levaduraMaV203

Tramado azul/blancoNo

FormatoTubo(s)

Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)

Mejora la calidad de los plásmidosNo

Tipo de productoCélulas de levadura

Cantidad4 x 0,1 mL

Condiciones de envíoHielo seco

EspecieS. cerevisiae

Línea de productosProQuest

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador ultrafrío (entre -68 y -85 °C).

Preguntas frecuentes

Does the ProQuest Two-Hybrid System yeast strain, MaV203 have slow growth compared to other commonly used laboratory Saccharomyces cerevisiae strains?

To avoid interference of endogenous GAL4 and GAL80 proteins, MaV203 must carry deletions of the GAL4 and GAL80 genes. As a result of deletion of these two genes, MaV203 cells grow more slowly compared to yeast strains containing the wild-type version of these genes. Please note that the growth rate will depend on the type of growth medium used (e.g., YPD versus SC drop-out media).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are the MaV203 yeast cells that come with the ProQuest Two-Hybrid System ready for transformation?

No, the MaV203 cells are provided as a glycerol stock. Cells from this stock need to be streaked on a YPD plate for growth at 30 degrees C. Resulting colonies can be used to prepare competent cells for transformation as per the ProQuest Two-Hybrid System manual. Alternatively, ready-to-use MaV203 Competent Yeast Cells, Subcloning Scale (Cat. No. 11445-012) or Library Scale (Cat. No. 11281-011) are available for purchase from Thermo Fisher Scientific.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I sequenced one of your vectors after PCR amplification and observed a difference from what is provided online (or in the manual). Should I be concerned?

Our vectors have not been completely sequenced. Your sequence data may differ when compared to what is provided. Known mutations that do not affect the function of the vector are annotated in public databases.

Are your vectors routinely sequenced?

No, our vectors are not routinely sequenced. Quality control and release criteria utilize other methods.

How was the reference sequence for your vectors created?

Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources.