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Taq DNA Polymerase (Brasil)
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa.
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| Número de catálogo | N.º de reacciones |
|---|---|
| 11615010 | 500 reacciones |
| 11615036 | 100 reacciones |
| 11615025 | 1000 reacciones |
| 11615044 | 5000 reacciones |
Número de catálogo 11615010
Precio (CLP)
92.727
Each
N.º de reacciones:
500 reacciones
Precio (CLP)
92.727
Each
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity (see figure).
Taq DNA Polymerase native enzyme is purified from Thermus aquaticus YT1. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Features
- Choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Especificaciones
Fidelidad (frente a Taq)1X
Inicio en calienteNo
N.º de reacciones500 reacciones
Sobrante3'-A
PolimerasaTaq DNA Polymerase
Cantidad500 Units
Condiciones de envíoDry Ice
Tamaño (producto final)5 kb o menos
Material de partidaADN
Concentración5 U/μL
Para utilizar con (aplicación)RT-PCR
GC-Rich PCR PerformanceLow
Velocidad de reacciónEstándar
Unit SizeEach
Contenido y almacenamiento
• Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C