Kit de síntesis de ADNc SuperScript™ VILO™
Kit de síntesis de ADNc SuperScript™ VILO™
Citas y referencias (5)
Invitrogen™

Kit de síntesis de ADNc SuperScript™ VILO™

El kit de síntesis de ADNc SuperScript VILO está diseñado para generar la primera cadena de ADNc para aplicaciones RT-qPCRMás información
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Número de catálogoN.º de reacciones
1175405050 reacciones
11754250250 reacciones
Número de catálogo 11754050
Precio (CLP)
714.852
Each
Añadir al carro de la compra
N.º de reacciones:
50 reacciones
Pedido a granel o personalizado
Precio (CLP)
714.852
Each
Añadir al carro de la compra
El kit de síntesis de ADNc SuperScript VILO está diseñado para generar la primera cadena de ADNc para aplicaciones RT-qPCR de dos pasos. El kit se suministra en un formato de 2 tubos con la mezcla de reacción VILO y una mezcla de enzimas en tubos separados. La mezcla enzimática contiene la transcriptasa inversa (RT) SuperScript III, una RT MMLV modificada genéticamente que ha reducido la actividad de la ARNasa H y ha mejorado la termoestabilidad para una síntesis de ADNc muy eficiente. El kit se puede utilizar para sintetizar ADNc desde un amplio intervalo de cantidades de ARN de entrada.

Nota: Para obtener un rendimiento superior de la síntesis de la primera cadena de ADNc en RT-qPCR de dos pasos, recomendamos la mezcla maestra SuperScript IV VILO o la mezcla maestra SuperScript IV VILO con enzima DNAasa ez. La mezcla maestra proporciona todos los componentes de reacción de síntesis de ADNc en un práctico formato de mezcla maestra de un tubo. Eleva la fiable tecnología VILO al siguiente nivel con la transcriptasa inversa altamente procesiva y termoestable SuperScript IV y un tampón aún más optimizado. La mezcla maestra permite una síntesis eficaz del ADNc a temperaturas más altas en menos tiempo y proporciona un rendimiento y sensibilidad superiores del ADNc incluso con una pureza subóptima o plantillas escasas.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Tipo de producto finalPrimera cadena de ADNc
FormatoKit
N.º de reacciones50 reacciones
Temperatura óptima de reacción42 °C
Cantidad50 reacciones
Formato de reacciónMezcla maestra o SuperMix
Tipo de reactivoTranscripción reversa
Transcriptasa inversaSuperScript III
Actividad de la ribonucleasa HReducido
Condiciones de envíoHielo seco
Tamaño (producto final)10 kb o menos
Material de partidaARN
TécnicaTranscripción reversa
Método de detecciónSonda de cebado
Para utilizar con (aplicación)Real Time PCR (qPCR)
GC-Rich PCR PerformanceAlto
Velocidad de reacción60 min
Unit SizeEach
Contenido y almacenamiento
• Mezcla de reacción VILO 5X que contiene cebadores aleatorios, dNTPs y MgCl2
• Mezcla de enzimas SuperScript III 10X que contiene Superscript III RT, inhibidor de ribonucleasa recombinante RNaseOUT y proteína auxiliar exclusiva

Almacenar en congelador (entre -5 a -30°C).

Preguntas frecuentes

How long can I store the cDNA from my reverse transcription step?

You can store your cDNA at 2-6 degrees C for up to 24 hours. For long-term storage, store the cDNA at -15 to -25 degrees C and add EDTA to a final concentration of 1 mM to prevent degradation.

What is the difference between SuperScript III RT and the RT in the SuperScript VILO kit?

The SuperScript VILO cDNA Synthesis Kit contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript

The SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) contains a mix of SuperScript III RT and helper proteins which help to increase the efficiency of the reverse transcription reaction and thus improve yield. The RT in the SuperScript VILO kit is active at 42 degrees C due to the helper proteins.

How much of the first-strand cDNA reaction should I load for PCR?

While the volume is dependent on the starting amount of RNA used for the first-strand synthesis and the abundance of the target gene, we'd recommend starting with 10% of the first-strand reaction for your PCR reaction.

In what concentration range of total RNA does the SuperScript VILO cDNA Synthesis Kit (Cat. No. 11754050) work?

  • Lower limit of RNA concentration: 1.0 pg
  • Upper limit of RNA concentration: 2.5 µg


    • Find additional tips, troubleshooting help, and resources within our Reverse Transcription and RACE Support Center

    What is the RNA input for the Oncomine Focus Assay, Chef-Ready Library kit?

    Each reverse transcription reaction requires 10 ng of DNase-treated total RNA (≥1.43 ng/µL).

    View all Kit de síntesis de ADNc SuperScript™ VILO™ FAQs

    Citations & References (5)

    Citations & References
    Abstract
    A useful approach to total analysis of RISC-associated RNA.
    Authors:Hayashida Y, Nishibu T, Inoue K, Kurokawa T,
    Journal:BMC Res Notes
    PubMed ID:19706194
    'ABSTRACT: BACKGROUND: Identifying the endogenous RNA induced silencing complex(RISC)-associated RNAs is essential for understanding the cellular regulatory networks by miRNAs. Recently, isolation of RISC-associated mRNAs using antibody was reported, but their method needs a large amount of initial materials. We tried to improve the protocol and constructed an efficient and ... More
    Transcriptome analysis of proliferating Arabidopsis endosperm reveals biological implications for the control of syncytial division, cytokinin signaling, and gene expression regulation.
    Authors:Day RC, Herridge RP, Ambrose BA, Macknight RC,
    Journal:Plant Physiol
    PubMed ID:18923020
    During the early stages of seed development, Arabidopsis (Arabidopsis thaliana) endosperm is syncytial and proliferates rapidly through repeated rounds of mitosis without cytokinesis. This stage of endosperm development is important in determining final seed size and is a model for studying aspects of cellular and molecular biology, such as the ... More
    T cells modulate Epstein-Barr virus latency phenotypes during infection of humanized mice.
    Authors:Heuts F, Rottenberg ME, Salamon D, Rasul E, Adori M, Klein G, Klein E, Nagy N,
    Journal:
    PubMed ID:24390326
    Human B cells, the main target of Epstein-Barr virus (EBV), can display several types of latent viral protein expression, denoted 0, I, IIa, IIb, or III. Of these, only type III expression induces proliferation of cells in vitro. These latency types are present at specific stages of infection and are ... More
    Decrease of miR-146b-5p in monocytes during obesity is associated with loss of the anti-inflammatory but not insulin signaling action of adiponectin.
    Authors:Hulsmans M, Van Dooren E, Mathieu C, Holvoet P,
    Journal:PLoS One
    PubMed ID:22393448
    Low adiponectin, a well-recognized antidiabetic adipokine, has been associated with obesity-related inflammation, oxidative stress and insulin resistance. Globular adiponectin is an important regulator of the interleukin-1 receptor-associated kinase (IRAK)/NF?B pathway in monocytes of obese subjects. It protects against inflammation and oxidative stress by inducing IRAK3. microRNA (miR)-146b-5p inhibits NF?B-mediated inflammation ... More
    Cyclin D1 splice variants: polymorphism, risk, and isoform-specific regulation in prostate cancer.
    Authors:Comstock CE, Augello MA, Benito RP, Karch J, Tran TH, Utama FE, Tindall EA, Wang Y, Burd CJ, Groh EM, Hoang HN, Giles GG, Severi G, Hayes VM, Henderson BE, Le Marchand L, Kolonel LN, Haiman CA, Baffa R, Gomella LG, Knudsen ES, Rui H, Henshall SM, Sutherland RL, Knudsen KE,
    Journal:Clin Cancer Res
    PubMed ID:19706803
    PURPOSE: Alternative CCND1 splicing results in cyclin D1b, which has specialized, protumorigenic functions in prostate not shared by the cyclin D1a (full length) isoform. Here, the frequency, tumor relevance, and mechanisms controlling cyclin D1b were challenged. EXPERIMENTAL DESIGN: First, relative expression of both cyclin D1 isoforms was determined in prostate ... More