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Citas y referencias (10)
Invitrogen™
Gateway™ pYES-DEST52 Vector
El vector de destino pYES2-DEST52 Gateway™ está diseñado para la clonación rápida con un clon de entrada Gateway™ utilizando laMás información
| Número de catálogo | Cantidad |
|---|---|
| 12286019 | 6 μg |
Número de catálogo 12286019
Precio (CLP)
620.704
Each
Cantidad:
6 μg
Precio (CLP)
620.704
Each
El vector de destino pYES2-DEST52 Gateway™ está diseñado para la clonación rápida con un clon de entrada Gateway™ utilizando la recombinación específica de sitio de fago lambda y la expresión posterior en Saccharomyces cerevisiae. Como parte de la colección de vectores YES™, pYES2-DEST52 lleva las secuencias promotoras y potenciadoras del gen GAL1 para expresión regulada en S.cerevisiae.
PYES2-DEST52 incluye:
• Origen de la replicación de 2 µ para el mantenimiento de alto número de copias
Gen para la selección auxotrófica en medio mínimo económico
• Etiquetas de epítopo C-terminal V5 y polihistidina (6xHis) para una detección y purificación eficaces
Sitios tR para una recombinación eficaz con cualquier vector de entrada Gateway™ flanqueado por attL
PYES2-DEST52 incluye:
• Origen de la replicación de 2 µ para el mantenimiento de alto número de copias
Gen para la selección auxotrófica en medio mínimo económico
• Etiquetas de epítopo C-terminal V5 y polihistidina (6xHis) para una detección y purificación eficaces
Sitios tR para una recombinación eficaz con cualquier vector de entrada Gateway™ flanqueado por attL
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Resistencia bacteriana a los antibióticosAmpicilina (AMPR)
Tipo de productoVector de expresión de destino del sistema Gateway
Cantidad6 μg
VectorpYES, pDEST
Método de clonaciónGateway
Línea de productosGateway
PromotorGAL1
Etiqueta de proteínaEtiqueta His (6x), Etiqueta de epítopo V5
Unit SizeEach
Contenido y almacenamiento
El vector de destino pYES2-DEST52 Gateway™ se suministra superenrollado y liofilizado. Almacenar a – 20 °C. Se garantiza la estabilidad de este vector durante seis meses si se almacena adecuadamente.
Preguntas frecuentes
Can I perform the single-step protocol for the BP/LR Clonase reaction using BP Clonase enzyme and LR Clonase enzyme instead of BP Clonase II enzyme and LR Clonase II enzyme?
Do you have a recommended single-step protocol for BP/LR recombination?
How can I move my gene of interest from a Gateway-adapted expression clone to a new Destination vector as I have lost the entry clone?
Can I purchase the 5X LR Clonase buffer or 5X BP Clonase buffer separately?
Do you offer Gateway vectors for expression in plants?
Citations & References (10)
Citations & References
Abstract
Discovery of a gene family critical to wyosine base formation in a subset of phenylalanine-specific transfer RNAs.
Journal:J Biol Chem
PubMed ID:16162496
'A large number of post-transcriptional base modifications in transfer RNAs have been described (Sprinzl, M., Horn, C., Brown, M., Ioudovitch, A., and Steinberg, S. (1998) Nucleic Acids Res. 26, 148-153). These modifications enhance and expand tRNA function to increase cell viability. The intermediates and genes essential for base modifications in
Participation of an Endomembrane Cation/H+ Exchanger AtCHX20 in Osmoregulation of Guard Cells.
Journal:Plant Physiol
PubMed ID:17337534
'Guard cell movement is induced by environmental and hormonal signals that cause changes in turgor through changes in uptake or release of solutes and water. Several transporters mediating these fluxes at the plasma membrane have been characterized, however less is known about transport at endomembranes. CHX20, a member of a
Identification of a receptor-independent activator of G protein signaling (AGS8) in ischemic heart and its interaction with Gbetagamma.
Journal:Proc Natl Acad Sci U S A
PubMed ID:16407149
'As part of a broader effort to identify postreceptor signal regulators involved in specific diseases or organ adaptation, we used an expression cloning system in Saccharomyces cerevisiae to screen cDNA libraries from rat ischemic myocardium, human heart, and a prostate leiomyosarcoma for entities that activated G protein signaling in the
Isolation and characterization of cDNAs encoding an enzyme with glucosyltransferase activity for cyclo-DOPA from four o'clocks and feather cockscombs.
Journal:Plant Cell Physiol
PubMed ID:15695438
cDNAs encoding an enzyme with UDP-glucose:cyclo-DOPA 5-O-glucosyltransferase activity were isolated from four o'clocks and feather cockscombs. Phylogenetic analysis of the amino acid sequences deduced from the cDNAs show that they represent a single subclade distinct from those of other phenylpropanoid and flavonoid glucosyltransferases. Changes in the amount of transcripts of
Molecular and Biochemical Characterization of a Novel Intracellular Invertase from Aspergillus niger with Transfructosylating Activity.
Journal:Eukaryot Cell
PubMed ID:17293485
A novel sub-family of putative intracellular invertase enzymes (glycoside hydrolase family 32) has previously been identified in fungal genomes. Here we report phylogenetic, molecular and biochemical characteristics of SucB, one of two novel intracellular invertases identified in Aspergillus niger. The sucB gene was expressed in Escherichia coli and an invertase