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Gibco™
DMEM/F-12, polvo, HEPES
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many differentMás información
| Número de catálogo | Cantidad |
|---|---|
| 12400024 | 10 x1 L |
Número de catálogo 12400024
Precio (CLP)
114.018
Each
Cantidad:
10 x1 L
Precio (CLP)
114.018
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM/F-12 is modified as follows:
With: L-glutamine, HEPES, Phenol Red
Without: Sodium Bicarbonate
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH. Powder forms of Gibco™ cell culture media require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).
Please note that the shelf life on delivered products varies. This product has a minimum shelf life of 6 months at time of shipment. For more information regarding product shelf life, including details on specific product lots, please contact Customer Service.
Especificaciones
Línea de célulasMDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoDMEM (Dulbecco's Modified Eagle Medium)/F-12
Cantidad10 x1 L
Duración de almacenamiento36 Months From Date of Manufacture
Condiciones de envíoTemperatura ambiente
ClasificaciónAnimal Origin-free
FormularioPolvo
Serum LevelSuplementos de suero estándar
EsterilidadEstéril con filtro
Con aditivosHigh Glucose, Glutamine, HEPES, Phenol Red, Sodium Pyruvate
Sin aditivosNo Sodium Bicarbonate
Unit SizeEach
Contenido y almacenamiento
Storage conditions: 2-8°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture