Citas y referencias (5)

Gibco™

Suplemento AmnioMAX™ C-100

Name: Suplemento AmnioMAX™ C-100
SKU: 12556023
Price: 389.446 CLP

Una parte del kit utilizado para preparar el medio completo Gibco AmnioMAX C-100 suplementado por completo y desarrollado para cultivar células del líquido amniótico humano a corto plazo para estudios citogenéticos y procedimientos de diagnóstico in vitro

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Número de catálogo	Cantidad
12556023	75 mL
12556015	15 mL

2 opciones

Número de catálogo 12556023

Precio (CLP)

389.446

Each

Cantidad:

75 mL

Precio (CLP)

389.446

Each

Gibco™ AmnioMAX™ C-100 Supplement is part of a kit used to prepare a fully-supplemented Gibco™ AmnioMAX™ C-100 Complete Medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Formato de uso sencillo: El medio completo AmnioMAX C-100 Gibco es un kit de dos partes para la preparación de un medio completo que no requiere suplementos adicionales. El medio completo puede almacenarse entre 2 y 8 °C durante un máximo de diez días

Calidad y pruebas de rendimiento
Cada lote del medio completo AmnioMAX C-100 Gibco se ha probado en un laboratorio de citogenética de referencia certificado en EE. UU. para garantizar un rendimiento superior en todo momento
Las muestras de líquido amniótico humano primario agrupadas se cultivan durante seis días en el medio completo AmnioMAX C-100 Gibco antes de medir el número total de colonias y el número total de colonias mitóticas
Además, cada lote se somete a pruebas de esterilidad, pH y osmolalidad
Formulación única y optimizada
El medio completo AmnioMAX C-100 Gibco contiene suero bovino fetal (SBF), gentamicina y L-glutamina para maximizar la fijación y el crecimiento celular
Cuenta con un sistema de amortiguación mejorado que proporciona una mayor estabilidad del pH durante las manipulaciones de los cultivos

Líquido amniótico y cultivo de vellosidades coriónicas, análisis celular, cultivo celular, proliferación celular, viabilidad celular, proliferación y función, clínica, investigación clínica y traslacional, citogenética, cultivo de células de mamíferos

Diagnóstico in vitro.

Especificaciones

Características ecológicasACT Ecolabel (Silver)

Línea de productosAmnioMAX

Cantidad75 mL

Duración de almacenamiento16 meses

Condiciones de envíoHielo seco

FormularioCongelado

Tipo de productoSuplemento

EsterilidadEstéril con filtro

Sterilization MethodSterile-filtered

Con aditivosGlutamina

Unit SizeEach

Contenido y almacenamiento

Condiciones de almacenamiento: De – 5 a – 20 °C
Condiciones de envío: Congelado
Vida útil: 16 meses a partir de la fecha de fabricación. Use el medio completo en un plazo de 7 a 10 días una vez abierto.

Citations & References (5)

Citations & References

Abstract

Induced pluripotent stem cells offer new approach to therapy in thalassemia and sickle cell anemia and option in prenatal diagnosis in genetic diseases.

Authors:Ye L, Chang JC, Lin C, Sun X, Yu J, Kan YW,

Journal:Proc Natl Acad Sci U S A

PubMed ID:19482945

'The innovation of reprogramming somatic cells to induced pluripotent stem cells provides a possible new approach to treat beta-thalassemia and other genetic diseases such as sickle cell anemia. Induced pluripotent stem (iPS) cells can be made from these patients'' somatic cells and the mutation in the beta-globin gene corrected by ... More

Reactivation of XIST in normal fibroblasts and a somatic cell hybrid: abnormal localization of XIST RNA in hybrid cells.

Authors:Hansen RS, Canfield TK, Stanek AM, Keitges EA, Gartler SM,

Journal:Proc Natl Acad Sci U S A

PubMed ID:9560241

'The XIST gene, expressed only from the inactive X chromosome, is a critical component of X inactivation. Although apparently unnecessary for maintenance of inactivation, XIST expression is thought to be sufficient for inactivation of genes in cis even when XIST is located abnormally on another chromosome. This repression appears to ... More

Specific tumour-associated methylation in normal human term placenta and first-trimester cytotrophoblasts.

Authors:Novakovic B, Rakyan V, Ng HK, Manuelpillai U, Dewi C, Wong NC, Morley R, Down T, Beck S, Craig JM, Saffery R,

Journal:Mol Hum Reprod

PubMed ID:18708652

'Human placentation displays many similarities with tumourigenesis, including rapid cell division, migration and invasion, overlapping gene expression profiles and escape from immune detection. Recent data have identified promoter methylation in the Ras association factor and adenomatous polyposis coli tumour suppressor genes as part of this process. However, the extent of ... More

Placenta-specific methylation of the vitamin D 24-hydroxylase gene: implications for feedback autoregulation of active vitamin D levels at the fetomaternal interface.

Authors:Novakovic B, Sibson M, Ng HK, Manuelpillai U, Rakyan V, Down T, Beck S, Fournier T, Evain-Brion D, Dimitriadis E, Craig JM, Morley R, Saffery R,

Journal:J Biol Chem

PubMed ID:19237542

'Plasma concentrations of biologically active vitamin D (1,25-(OH)(2)D) are tightly controlled via feedback regulation of renal 1alpha-hydroxylase (CYP27B1; positive) and 24-hydroxylase (CYP24A1; catabolic) enzymes. In pregnancy, this regulation is uncoupled, and 1,25-(OH)(2)D levels are significantly elevated, suggesting a role in pregnancy progression. Epigenetic regulation of CYP27B1 and CYP24A1 has previously ... More

Development of transgenic cloned pig models of skin inflammation by DNA transposon-directed ectopic expression of human ß1 and a2 integrin.

Authors:Staunstrup NH, Madsen J, Primo MN, Li J, Liu Y, Kragh PM, Li R, Schmidt M, Purup S, Dagnæs-Hansen F, Svensson L, Petersen TK, Callesen H, Bolund L, Mikkelsen JG,

Journal:PLoS One

PubMed ID:22590584

Integrins constitute a superfamily of transmembrane signaling receptors that play pivotal roles in cutaneous homeostasis by modulating cell growth and differentiation as well as inflammatory responses in the skin. Subrabasal expression of integrins a2 and/or ß1 entails hyperproliferation and aberrant differentiation of keratinocytes and leads to dermal and epidermal influx ... More