Suplemento B-27™ (50X), menos vitamina A
Suplemento B-27™ (50X), menos vitamina A
Citas y referencias (2)
Gibco™

Suplemento B-27™ (50X), menos vitamina A

La variación del suplemento de cultivo celular neuronal más citado, el suplemento B-27 sin vitamina A es una versión personalizadaMás información
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Número de catálogoCantidad
1258701010 mL
12587001100 mL
Número de catálogo 12587010
Precio (CLP)
187.411
Each
Añadir al carro de la compra
Cantidad:
10 mL
Precio (CLP)
187.411
Each
Añadir al carro de la compra
La variación del suplemento de cultivo celular neuronal más citado, el suplemento B-27 sin vitamina A es una versión personalizada del suplemento B-27 sin vitamina A original (acetato de retinilo). El suplemento B-27 sin vitamina A es un suplemento sin suero ideal para el cultivo de progenitor neural y células madre, ya sea como neuroesferas en cultivo en suspensión o en monocapa adherente, sin inducir diferenciación. El suplemento B-27 sin vitamina A se suministra como un líquido 50X y se ha diseñado para usar con medios Neurobasal o Neurobasal-A para realizar cultivos celulares de neuronas sin necesidad de una capa de alimentación de astrocitos.

El suplemento B-27 es adecuado para usar en la mayoría de las aplicaciones de células neuronales. Para otras aplicaciones, ofrecemos formulaciones alternativas del Suplemento B-27 y medios Neurobasal.

Para protocolos establecidos que usen el suplemento B-27, acceda a nuestro manual de protocolos de neurobiología Gibco.

Vea las publicaciones que utilizan productos de neurobiología Gibco en nuestra CellCite Gibco.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Tipo de célulaCélulas progenitoras neuronales
Concentración50 X
Tipo de cultivoCultivo de células de mamíferos
Línea de productosB-27
Cantidad10 mL
Nivel de sueroSin suero
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FuenteSin suero
Probado paraEndotoxinas, rendimiento, esterilidad, micoplasma
ClasificaciónSin suero
FormularioLíquido
Tipo de productoSuplemento de cultivo celular
EsterilidadEstéril
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador (de – 5 a – 20°C) y proteger de la luz.

Preguntas frecuentes

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I thaw B-27 Supplements in a 37 degrees C water bath or incubator?

We do not recommend thawing at 37 degrees C. Best results are observed upon thawing at room temperature for approximately 2 hrs or overnight at 2-8 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Suplemento B-27™ (50X), menos vitamina A FAQs

Citations & References (2)

Citations & References
Abstract
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Type I astrocytes and microglia induce a cytokine response in an encephalitic murine coronavirus infection.
Authors:Lavi E, Cong L
Journal:Exp Mol Pathol
PubMed ID:32454103
'The pathogenesis of viral infections involves an immune response by cytokines, causing a deleterious effect on organ function, in addition to tissue destruction due to viral replication. Clinical symptoms and laboratory findings of the human coronavirus disease COVID-19, caused by the novel coronavirus SARS CoV-2, indicate cytokine involvement. Our laboratory ... More