Extracto de pituitaria bovina (BPE)

La concentración de proteínas de cada lote de extracto de pituitaria bovina se determina por el método de Bradford. CadaMás información

Número de catálogo	Cantidad
13028014	25 mg

Número de catálogo 13028014

Precio (CLP)

Cantidad:

25 mg

La concentración de proteínas de cada lote de extracto de pituitaria bovina se determina por el método de Bradford. Cada vial de BPE contiene al menos de 25 mg de proteína.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas de mamíferos

Tipo de cultivoCultivo de células de mamífero

Cantidad25 mg

Duración de almacenamiento18 meses

Condiciones de envíoHielo seco

FormularioLíquido

Tipo de productoExtracto de hipófisis bovina

pHentre 6 y 8

Unit SizeEach

Contenido y almacenamiento

Almacenar en congelador (de – 5 a – 30 °C).

Preguntas frecuentes

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (1)

Citations & References

Abstract

Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling.

Authors:Utani A, Momota Y, Endo H, Kasuya Y, Beck K, Suzuki N, Nomizu M, Shinkai H,

Journal:J Biol Chem

PubMed ID:12826666

'The LG4 module of the laminin alpha 3 chain (alpha 3 LG4), a component of epithelial-specific laminin-5, has cell attachment activity and binds syndecan (Utani, A., Nomizu, M., Matsuura, H., Kato, K., Kobayashi, T., Takeda, U., Aota, S., Nielsen, P. K., and Shinkai, H. (2001) J. Biol. Chem. 276, 28779-28788). ... More