Penicilina-estreptomicina (5.000 U/ml)
Penicilina-estreptomicina (5.000 U/ml)
Citas y referencias (23)
Gibco™

Penicilina-estreptomicina (5.000 U/ml)

Los antibióticos penicilina y estreptomicina se usan para impedir la contaminación bacteriana de los cultivos celulares debido a su eficazMás información
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Número de catálogoCantidad
15070063100 mL
Número de catálogo 15070063
Precio (CLP)
25.787
Each
Añadir al carro de la compra
Cantidad:
100 mL
Precio (CLP)
25.787
Each
Añadir al carro de la compra
Los antibióticos penicilina y estreptomicina se usan para impedir la contaminación bacteriana de los cultivos celulares debido a su eficaz acción combinada contra bacterias grampositivas y gramnegativas. La penicilina se purificó en un principio a partir de los hongos Penicillium y actúa interfiriendo directamente con el volumen de la pared celular bacteriana, e indirectamente mediante la activación de la liberación de enzimas que alteran aún más la pared celular. La estreptomicina fue originalmente purificada a partir de Streptomyces griseus. Actúa mediante la creación de una unión a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y la muerte de bacterias susceptibles. Esta solución contiene 5000 unidades/ml de penicilina y 5000 µg/ml de estreptomicina.

Ofrecemos una amplia gama de antibióticos y antimicóticos tanto en formato en polvo como líquido. Consulte la lista completa o busque productos para:

• Control de la contaminación
• Selección de bacterias y eucariotas

Consulte las concentraciones de trabajo recomendadas para la selección de antibióticos.

Obtenga más información sobre el uso de antibióticos y antimicóticos en cultivo celular y revise las directrices para la descontaminación de cultivos.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración100X
Tipo de cultivoCultivo celular de mamíferos, cultivo celular de insectos
Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular
Cantidad100 mL
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FormularioLíquido
Tipo de productoPenicilina-estreptomicina
EsterilidadEstéril con filtro
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De – 5 °C a – 20 °C
Condiciones de envío: Hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

My Penicillin-Streptomycin solution is not colorless. Is this normal?

Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (23)

Citations & References
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Authors:Mueller Cornelius; Baudler Stephanie; Welzel Hilke; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are ... More
Directed differentiation of pancreatic δ cells from human pluripotent stem cells.
Authors:Chen L,Wang N,Zhang T,Zhang F,Zhang W,Meng H,Chen J,Liao Z,Xu X,Ma Z,Xu T,Liu H
Journal:Nature communications
PubMed ID:39068220
Dysfunction of pancreatic δ cells contributes to the etiology of diabetes. Despite their important role, human δ cells are scarce, limiting physiological studies and drug discovery targeting δ cells. To date, no directed δ-cell differentiation method has been established. Here, we demonstrate that fibroblast growth factor (FGF) 7 promotes pancreatic ... More
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Authors:Wiedemann A, Rosselin M, Mijouin L, Bottreau E, Velge P,
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion ... More
A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.
Authors: Watters Jyoti J; Sommer Julie A; Pfeiffer Zachary A; Prabhu Usha; Guerra Alma N; Bertics Paul J;
Journal:J Biol Chem
PubMed ID:11786532
'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in ... More
Electrophoretic profiling of both RNA and protein from a single 250-pL sample.
Authors: Zabzdyr Jennifer L; Lillard Sheri J;
Journal:Anal Chem
PubMed ID:11985318
'A novel approach is described that uses capillary electrophoresis (CE) to electrophoretically sample and separate both protein and RNA from a single injected plug of cell lysate. A 250-pL sample of lysate from Chinese hamster ovary cells (9.6 x 10(7) cells/mL) was hydrodynamically injected into a capillary containing a Tris-based ... More
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