Antibiótico-antimicótico (100X)
Antibiótico-antimicótico (100X)
Gibco™

Antibiótico-antimicótico (100X)

El antibiótico-antimicótico Gibco™ se usa para prevenir la contaminación por hongos y bacterias. Esta solución contiene 10.000 unidades/ml de penicilina,Más información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidad
15240062100 mL
1524009620 mL
1524011220 x 100 mL
Número de catálogo 15240062
Precio (CLP)
51.665
Each
Añadir al carro de la compra
Cantidad:
100 mL
Precio (CLP)
51.665
Each
Añadir al carro de la compra
El antibiótico-antimicótico Gibco™ se usa para prevenir la contaminación por hongos y bacterias. Esta solución contiene 10.000 unidades/ml de penicilina, 10.000 µg/ml de estreptomicina y 25 µg/ml de anfotericina B Gibco. Los antibióticos penicilina y estreptomicina impiden la contaminación bacteriana de los cultivos celulares debido a su eficaz acción combinada contra bacterias grampositivas y gramnegativas. La anfotericina B impide la contaminación fúngica de cultivos celulares debido a su inhibición de hongos y levaduras multicelulares.

La penicilina se purificó en un principio a partir del hongo Penicillium y actúa mediante una interferencia directa con el volumen de la pared celular bacteriana e indirecta mediante la activación de la liberación de enzimas que alteran aún más la pared celular. La estreptomicina fue originalmente purificada a partir de Streptomyces griseus. Actúa mediante la creación de una unión a la subunidad 30S del ribosoma bacteriano, lo que inhibe la síntesis de proteínas y la muerte de las bacterias susceptibles. La anfotericina B es un agente antimicótico que impide el crecimiento de hongos y levaduras al provocar un aumento de la permeabilidad de la membrana plasmática fúngica.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración100X
Tipo de cultivoCultivo de células de mamífero
Cantidad100 mL
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FormularioLíquido
Tipo de productoAntibiótico y antimicótico
EsterilidadEstéril con filtro
Sterilization MethodSterile-filtered
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De – 5 °C a – 20 °C
Condiciones de envío: Hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

Can I use pen/strep or other antibiotics for episomal reprogramming of cells?

The use of antibiotics is not recommended during episomal reprogramming, especially Fungizone antimycotic. If antibiotics must be used during the reprogramming process, pen/strep could be used. For established iPSCs (after reprogramming is complete), either antibiotic/antimycotic or pen/strep should be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (13)

Citations & References
Abstract
Common variation at 6q16 within HACE1 and LIN28B influences susceptibility to neuroblastoma.
Authors:Diskin SJ, Capasso M, Schnepp RW, Cole KA, Attiyeh EF, Hou C, Diamond M, Carpenter EL, Winter C, Lee H, Jagannathan J, Latorre V, Iolascon A, Hakonarson H, Devoto M, Maris JM,
Journal:Nat Genet
PubMed ID:22941191
Neuroblastoma is a cancer of the sympathetic nervous system that accounts for approximately 10% of all pediatric oncology deaths. Here, we report a genome-wide association study of 2,817 neuroblastoma cases and 7,473 controls. We identified two new associations at 6q16, the first within HACE1 (rs4336470; combined P=2.7×10(-11); odds ratio 1.26, ... More
Patient-derived glioblastoma organoids as real-time avatars for assessing responses to clinical CAR-T cell therapy
Authors:Meghan Logun, Xin Wang, Yusha Sun, Stephen J Bagley, Nannan Li, Arati Desai, Daniel Y Zhang, MacLean P Nasrallah, Emily Ling-Lin Pai, Bike Su Oner, Gabriela Plesa, Donald Siegel, Zev A Binder, Guo-Li Ming, Hongjun Song, Donald M O'Rourke
Journal:Cell Stem Cell
PubMed ID:39657679
Patient-derived tumor organoids have been leveraged for disease modeling and preclinical studies but rarely applied in real time to aid with interpretation of patient treatment responses in clinics. We recently demonstrated early efficacy signals in a first-in-human, phase 1 study of dual-targeting chimeric antigen receptor (CAR)-T cells (EGFR-IL13Rα2 CAR-T cells) ... More
Non-viral-mediated gene transfer of OX40 ligand for tumor immunotherapy.
Authors:Rakitina OA,Kuzmich AI,Bezborodova OA,Kondratieva SA,Pleshkan VV,Zinovyeva MV,Didych DA,Sass AV,Snezhkov EV,Kostina MB,Koksharov MO,Alekseenko IV
Journal:Frontiers in immunology
PubMed ID:39007148
BACKGROUND: Immune checkpoint blockade (ICB) is rapidly becoming a standard of care in the treatment of many cancer types. However, the subset of patients who respond to this type of therapy is limited. Another way to promote antitumoral immunity is the use of immunostimulatory molecules, such as cytokines or T ... More
Protocol to generate human liver spheroids to study liver fibrosis induced by metabolic stress.
Authors:Kim HY,Lee W,Liu X,Jang H,Sakane S,Carvalho-Gontijo Weber R,Diggle K,Kerk SA,Metallo CM,Kisseleva T,Brenner DA
Journal:STAR protocols
PubMed ID:38833372
Currently, there is no effective treatment for obesity and alcohol-associated liver diseases, partially due to the lack of translational human models. Here, we present a protocol to generate 3D human liver spheroids that contain all the liver cell types and mimic “livers in a dish.” We describe strategies to induce ... More
Single-Cell Transcriptome Atlas of Murine Endothelial Cells.
Authors:Kalucka J, de Rooij LPMH, Goveia J, Rohlenova K, Dumas SJ, Meta E, Conchinha NV, Taverna F, Teuwen LA, Veys K, García-Caballero M, Khan S, Geldhof V, Sokol L, Chen R, Treps L, Borri M, de Zeeuw P, Dubois C, Karakach TK, Falkenberg KD, Parys M, Yin X, Vinckier S, Du Y, Fenton RA, Schoonjans L, Dewerchin M, Eelen G, Thienpont B, Lin L, Bolund L, Li X, Luo Y, Carmeliet P
Journal:Cell
PubMed ID:32059779
'The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7' ... More