Antibiótico-antimicótico (100X)
Antibiótico-antimicótico (100X)
Gibco™

Antibiótico-antimicótico (100X)

El antibiótico-antimicótico Gibco™ se usa para prevenir la contaminación por hongos y bacterias. Esta solución contiene 10.000 unidades/ml de penicilina,Más información
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Número de catálogoCantidad
1524009620 mL
15240062100 mL
1524011220 x 100 mL
Número de catálogo 15240096
Precio (CLP)
19.811
Each
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Cantidad:
20 mL
Precio (CLP)
19.811
Each
Añadir al carro de la compra
El antibiótico-antimicótico Gibco™ se usa para prevenir la contaminación por hongos y bacterias. Esta solución contiene 10.000 unidades/ml de penicilina, 10.000 µg/ml de estreptomicina y 25 µg/ml de anfotericina B Gibco. Los antibióticos penicilina y estreptomicina impiden la contaminación bacteriana de los cultivos celulares debido a su eficaz acción combinada contra bacterias grampositivas y gramnegativas. La anfotericina B impide la contaminación fúngica de cultivos celulares debido a su inhibición de hongos y levaduras multicelulares.

La penicilina se purificó en un principio a partir del hongo Penicillium y actúa mediante una interferencia directa con el volumen de la pared celular bacteriana e indirecta mediante la activación de la liberación de enzimas que alteran aún más la pared celular. La estreptomicina fue originalmente purificada a partir de Streptomyces griseus. Actúa mediante la creación de una unión a la subunidad 30S del ribosoma bacteriano, lo que inhibe la síntesis de proteínas y la muerte de las bacterias susceptibles. La anfotericina B es un agente antimicótico que impide el crecimiento de hongos y levaduras al provocar un aumento de la permeabilidad de la membrana plasmática fúngica.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración100X
Tipo de cultivoCultivo de células de mamífero
Cantidad20 mL
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FormularioLíquido
Tipo de productoAntibiótico y antimicótico
EsterilidadEstéril con filtro
Sterilization MethodSterile-filtered
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De – 5 a – 20 °C
Condiciones de envío: Hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

Can I add antibiotics to OptiPRO SFM?

You can supplement OptiPRO SFM with 5mL/L Antibiotic-Antimycotic (Cat. No. 15240096)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I use antibiotics such as Pen-Strep or Antibiotic-Antimycotic (100X) when culturing Sf9 insect cells?

Yes, however, we do not recommend adding antibiotics to the medium at the time of thawing. Thaw cells into medium without antibiotic and allow the cells to recover from the thawing process. After that, you can add antibiotics by diluting into the medium.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the solvent used in Antibiotic-Antimycotic (100X)?

The components are in a 0.85% saline solution.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the components of Antibiotic-Antimycotic (100X)?

This solution contains 10,000 units/mL of penicillin, 10,000 µg/mL of streptomycin, and 25 µg/mL of Gibco Amphotericin B.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition for Antibiotic-Antimycotic (100X)?

The recommended condition is storage at -5 to -20 degrees C and when stored as recommended, the shelf life is 12 months from the date of shipment.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (6)

Citations & References
Abstract
Bombyxin is a growth factor for wing imaginal disks in Lepidoptera.
Authors: Nijhout H Frederik; Grunert Laura W;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12429853
'The mechanisms that control the growth rate of internal tissues during postembryonic development are poorly understood. In insects, the growth rate of imaginal disks varies with nutrition and keeps pace with variation in somatic growth. We describe here a mechanism by which the growth of wing imaginal disks is controlled. ... More
Apoptosis induces efflux of the mitochondrial matrix enzyme deoxyguanosine kinase.
Authors:Jüllig M, Eriksson S,
Journal:J Biol Chem
PubMed ID:11294860
Deoxyguanosine kinase (dGK) initiates the salvage of purine deoxynucleosides in mitochondria and is a key enzyme in mitochondrial DNA precursor synthesis. The active form of the enzyme is a 60-kDa protein normally located in the mitochondrial matrix. Here we describe the subcellular distribution of dGK during apoptosis in human epithelial ... More
Hypoxia and Nitric Oxide Treatment Confer Tolerance to Glucose Starvation in a 5'-AMP-activated Protein Kinase-dependent Manner.
Authors: Esumi Hiroyasu; Izuishi Kunihiko; Kato Kazuyoshi; Hashimoto Koichi; Kurashima Yukiko; Kishimoto Atsuhiro; Ogura Tsutomu; Ozawa Takayuki;
Journal:J Biol Chem
PubMed ID:12091379
Hypoxia is a critical event for higher organisms, and cells and tissues react by increasing the oxygen supply by vasodilatation, angiogenesis, and erythropoiesis and maintaining cellular energy by increasing glycolysis and inhibiting anabolic pathways. Stimulation of glycolysis has been regarded as the main response that increases energy production during hypoxia; ... More
Metabolic stress activates an ERK/hnRNPK/DDX3X pathway in pancreatic ß cells.
Authors:Good AL, Haemmerle MW, Oguh AU, Doliba NM, Stoffers DA
Journal:Mol Metab
PubMed ID:31178390
Pancreatic ß cell failure plays a central role in the development of type 2 diabetes (T2D). While the transcription factors shaping the ß cell gene expression program have received much attention, the post-transcriptional controls that are activated in ß cells during stress are largely unknown. We recently identified JUND as ... More
PNA Telomere and Centromere FISH Staining for Accurate Analysis of Radiation-Induced Chromosomal Aberrations.
Authors:Cartwright IM, Haskins JS, Kato TA
Journal:Methods Mol Biol
PubMed ID:31267424
Dicentric and centric ring chromosomes are used for radiation-induced damage analysis and biodosimetry after radiation exposure. However, Giemsa stain-based cytogenetic analysis is labor-intense and time-consuming. Moreover, the disadvantage of Giemsa based chromosome analysis is a potential poor reproducibility when researchers are not fully trained for analysis. These problems come from ... More