Anfotericina B
Anfotericina B
Citas y referencias (8)
Gibco™

Anfotericina B

La anfotericina B es el principio activo de Fungizone. Fungizone es una marca comercial de E.R. Squibb & Sons, LLC.Más información
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Número de catálogoCantidad
1529001820 ml
1529002650 mL
Número de catálogo 15290018
Precio (CLP)
33.737
Each
Añadir al carro de la compra
Cantidad:
20 ml
Precio (CLP)
33.737
Each
Añadir al carro de la compra
La anfotericina B es el principio activo de Fungizone. Fungizone es una marca comercial de E.R. Squibb & Sons, LLC. La anfotericina B es un antifúngico producido por Streptomyces nodosus. Previene el crecimiento de hongos al provocar un aumento de la permeabilidad de la membrana plasmática fúngica. Se une activamente a los esteroles y da lugar a la formación de poros. Se usa para prevenir la contaminación de cultivos de células con levaduras y hongos multicelulares. La anfotericina B Gibco contiene 250 µg de anfotericina B y 205 µg de deoxicolato sódico por ml de agua destilada. La concentración de trabajo recomendada oscila entre 0,25 y 2,50 µg/ml.

Fabricación según las buenas prácticas de fabricación actuales en dos instalaciones
Para mantener la continuidad de la cadena de suministro, fabricamos la anfotericina B Gibco en dos instalaciones, una ubicada en Grand Island, NY (EE. UU.) y la otra en Escocia (Reino Unido). Ambos sitios cumplen los requisitos de producción según las buenas prácticas de fabricación actuales, cuentan con la certificación ISO 13485 estándar y están registrados en la FDA como fabricantes de dispositivos médicos.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
ConcentraciónDe 0,25 a 2,5 μg/ml
Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular
Línea de productosFungizone
Cantidad20 ml
Duración de almacenamiento12 meses
Condiciones de envíoHielo seco
FormularioLíquido
Tipo de productoAntifúngico
EsterilidadEstéril con filtro
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De – 5 °C a – 20 °C
Condiciones de envío: Congelado en hielo seco
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How many freeze/thaw cycles do you recommend for Amphotericin B?

Amphotericin B can be freeze-thawed 2-3 times without appreciable loss of potency.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the stability of Amphotericin B when stored at 4 degrees C?

Amphotericin B in solution is stable at 2-8 degrees C for approximately 4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the difference between Fungizone and Amphotericin B?

Amphotericin B is the generic version of Fungizone. Fungizone is a trademark of E.R. Squibb & Sons, LLC.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (8)

Citations & References
Abstract
Use of human tissue explants to study human infectious agents.
Authors:Grivel JC, Margolis L,
Journal:Nat Protoc
PubMed ID:19197269
The study of human cell-cell and cell-pathogen interactions that occur in the context of complex tissue cytoarchitecture is critical for deciphering the mechanisms of many normal and pathogenic processes. This protocol describes methods for culturing and infecting explants of human tissues to study the pathogenesis of human infectious agents and ... More
Specific inhibition of ICAM-1 expression mediated by gene targeting with Triplex-forming oligonucleotides.
Authors:Besch R, Giovannangeli C, Kammerbauer C, Degitz K
Journal:J Biol Chem
PubMed ID:12080053
Selected sequences in the DNA double helix can be specifically recognized by oligonucleotides via hydrogen bonding interactions. The resulting triple helix can modulate DNA metabolism and especially interfere with transcription in a gene-specific manner. To explore the potential of triplex-forming oligonucleotides (TFOs) as gene repressors, a TFO was designed to ... More
Endogenous G Protein-coupled Receptor Kinase 6 Regulates M3 Muscarinic Acetylcholine Receptor Phosphorylation and Desensitization in Human SH-SY5Y Neuroblastoma Cells.
Authors: Willets Jonathon M; Challiss R A John; Nahorski Stefan R;
Journal:J Biol Chem
PubMed ID:11856737
We have previously shown that overexpression of G protein-coupled receptor kinase 6 (GRK6) enhanced the phosphorylation and desensitization of the endogenously expressed M(3) muscarinic acetylcholine (mACh) receptor in human SH-SY5Y neuroblastoma cells. In this study we have examined the potential role of endogenous GRK6 in the regulation of M(3) mACh ... More
CD40-mediated Activation of NF-kappa B in Airway Epithelial Cells.
Authors: Propst Stacie M; Estell Kim; Schwiebert Lisa M;
Journal:J Biol Chem
PubMed ID:12122011
We have reported previously that airway epithelial cells (AEC) express CD40 and that activation of this molecule stimulates the expression of inflammatory mediators, including the chemokine RANTES (regulated on activation normal T cell expressed and secreted). Because NF-kappaB regulates the expression of many inflammatory mediators, such as RANTES, we utilized ... More
A Dynamic Variation of Pulmonary ACE2 Is Required to Modulate Neutrophilic Inflammation in Response to
Authors:Sodhi CP, Nguyen J, Yamaguchi Y, Werts AD, Lu P, Ladd MR, Fulton WB, Kovler ML, Wang S, Prindle T, Zhang Y, Lazartigues ED, Holtzman MJ, Alcorn JF, Hackam DJ, Jia H
Journal:J Immunol
PubMed ID:31645418
'Angiotensin-converting enzyme 2 (ACE2) is a potent negative regulator capable of restraining overactivation of the renin-angiotensin system, which contributes to exuberant inflammation after bacterial infection. However, the mechanism through which ACE2 modulates this inflammatory response is not well understood. Accumulating evidence indicates that infectious insults perturb ACE2 activity, allowing for ... More
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