Gentamicina (50 mg/ml)
Gentamicina (50 mg/ml)
El producto real puede variar
Citas y referencias (13)
Gibco™

Gentamicina (50 mg/ml)

El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea.La gentamicina actúaMás información
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Número de catálogoCantidad
1575006010 mL
1575007810 x 10 mL
Número de catálogo 15750060
Precio (CLP)
83.660
Each
Añadir al carro de la compra
Cantidad:
10 mL
Precio (CLP)
83.660
Each
Añadir al carro de la compra
El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea.La gentamicina actúa uniéndose a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y a la muerte de las bacterias susceptibles.La gentamicina Gibco™ es eficaz contra una amplia variedad de bacterias grampositivas y gramnegativas, y se utiliza para la prevención de la contaminación bacteriana de cultivos celulares.La concentración de trabajo recomendada oscila entre 0,5 y 50 μg/ml.

Ofrecemos una amplia gama de antibióticos y antimicóticos tanto en polvo como en líquido.

Obtenga más información sobre el uso de antibióticos y antimicóticos en el cultivo celular y revise las directrices para la descontaminación de cultivos.

Fabricación según las prácticas correctas actuales en dos instalaciones
Gibco™ La gentamicina se fabrica en instalaciones que cumplen los principios de buenas prácticas actuales, ubicadas en Grand Island, Nueva York.Las instalaciones están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.Para mantener la continuidad de la cadena de suministro, podemos ofrecer un producto de gentamicina Gibco™ comparable que fabricamos en nuestras instalaciones de Escocia (15750-037).Estas instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.
Para uso exclusivo en investigación.No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración50 mg/mL
Almacenamiento recomendadoCondiciones de almacenamiento: De 15 °C a 30 °C
Condiciones de envío: Temperatura ambiente
Vida útil: 24 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
Filtrado estérilYes
Aplicación validadaPrevención de la contaminación del cultivo celular
Forma físicaLíquido
Cantidad10 mL
TipoGentamicina
Unit SizeEach

Preguntas frecuentes

Once Gentamicin (50 mg/mL; Cat. No. 15750060) is added to the cell culture medium, can this resulting medium, including gentamicin, be frozen and thawed before using it in cell culture without affecting the performance of the antibiotic?

Yes, gentamicin can be frozen in a prepared medium. However, we don't have any stability data on this process with media not made here in our facility. So, you would have to determine the effectiveness of the product upon thawing empirically.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (13)

Citations & References
Abstract
A comparison between different human hepatocyte models reveals profound differences in net glucose production, lipid composition and metabolism in vitro.
Authors:Bonanini F,Singh M,Yang H,Kurek D,Harms AC,Mardinoglu A,Hankemeier T
Journal:Experimental cell research
PubMed ID:38499143
Biosynthetic studies of A2E, a major fluorophore of retinal pigment epithelial lipofuscin.
Authors: Ben-Shabat Shimon; Parish Craig A; Vollmer Heidi R; Itagaki Yasuhiro; Fishkin Nathan; Nakanishi Koji; Sparrow Janet R;
Journal:J Biol Chem
PubMed ID:11756445
'We have examined questions related to the biosynthesis of A2E, a fluorophore that accumulates in retinal pigment epithelial cells with aging and in some retinal disorders. The use of in vitro preparations revealed that detectable levels of A2-PE, the A2E precursor, are formed within photoreceptor outer segments following light-induced release ... More
Growth-dependent Regulation of Mammalian Pyrimidine Biosynthesis by the Protein Kinase A and MAPK Signaling Cascades.
Authors: Sigoillot Frederic D; Evans David R; Guy Hedeel I;
Journal:J Biol Chem
PubMed ID:11872754
'The carbamoyl phosphate synthetase domain of the multifunctional protein CAD catalyzes the initial, rate-limiting step in mammalian de novo pyrimidine biosynthesis. In addition to allosteric regulation by the inhibitor UTP and the activator PRPP, the carbamoyl phosphate synthetase activity is controlled by mitogen-activated protein kinase (MAPK)- and protein kinase A ... More
The potent anti-HIV protein cyanovirin-N contains two novel carbohydrate binding sites that selectively bind to Man(8) D1D3 and Man(9) with nanomolar affinity: implications for binding to the HIV envelope protein gp120.
Authors: Bewley C A; Otero-Quintero S
Journal:J Am Chem Soc
PubMed ID:11457139
'Cyanovirin-N (CVN) is a monomeric 11 kDa cyanobacterial protein that potently inactivates diverse strains of human immunodeficiency virus (HIV) at the level of cell fusion by virtue of high affinity interactions with the surface envelope glycoprotein gp120. Several lines of evidence have suggested that CVN-gp120 interactions are in part mediated ... More
Isolation of carbohydrate-specific CD4(+) T cell clones from mice after stimulation by two model glycoconjugate vaccines.
Authors:Avci FY, Li X, Tsuji M, Kasper DL,
Journal:Nat Protoc
PubMed ID:23196974
Here we describe how to isolate carbohydrate-specific T cell clones (for which we propose the designation 'Tcarbs') after stimulation by two glycoconjugate vaccines. We describe how to prepare, purify and characterize two model glycoconjugate vaccines that can be used to generate Tcarbs. These glycoconjugate vaccines (GBSIII-OVA and GBSIII-TT) are synthesized ... More
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