Lipofectin™ Transfection Reagent
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Citas y referencias (54)
Invitrogen™

Lipofectin™ Transfection Reagent

El reactivo de transfección Lipofectin® es el reactivo de elección para la transfección de células endoteliales. El reactivo de transfecciónMás información
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Número de catálogoCantidad
182920374 mL
182920111 mL
Número de catálogo 18292037
Precio (CLP)
2.508.541
Each
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Cantidad:
4 mL
Precio (CLP)
2.508.541
Each
Añadir al carro de la compra
El reactivo de transfección Lipofectin® es el reactivo de elección para la transfección de células endoteliales. El reactivo de transfección Lipofectin® también es adecuado para transfectar ADN, ARN y oligonucleótidos en células de mamíferos, y ADN en protoplastos de plantas. También se ha demostrado que el reactivo Lipofectin® funciona bien, en combinación con el reactivo PLUS®, para la transfección de células HeLa. El reactivo de transfección Lipofectin® es una formulación liposoma 1:1 (p/p) del lípido catiónico N-[1-(2,3-dioleyloxy)propil]-n,n-trimetilamonio cloruro (DOTMA) y dioleoyl fofotidiletanolamina (DOPE) en agua filtrada por membrana.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (aplicación)Transfección
Compatibilidad de alto rendimientoNo compatible con alto rendimiento (manual)
IncluyeCuatro viales (1 ml cada uno) de reactivo de transfección Lipofectin
Línea de productosLipofectin
Tipo de productoReactivos para transfección
Cantidad4 mL
Compatible con sueroNo
Condiciones de envíoHielo húmedo
Tipo de célulaLíneas de células establecidas, células primarias, células difíciles de transfeccionar
FormatoPlaca de 6 pocillos, placa de 12 pocillos, placa de 24 pocillos, placa de 48 pocillos, placa de 96 pocillos, matraces
Tipo de muestraADN plasmídico
Transfection TechniqueTransfección basada en lípidos
Unit SizeEach
Contenido y almacenamiento
Cada unidad contiene uno o varios viales (1 ml) de reactivo. Almacenar a +4°C. Se garantiza la estabilidad del reactivo de transfección Lipofectin™ durante 6 meses si se almacena correctamente.

Preguntas frecuentes

I accidentally left my lipid reagent at room temperature. Can I still use it?

Yes, all of our lipid transfection reagents are stable at room temperature for months.

Find additional tips, troubleshooting help, and resources within our Lipid-Based Transfection Support Center.

Do you offer a reagent for the transfection of endothelial cells?

We recommend using the Lipofectamine 3000 Reagent for transfection of endothelial cells.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

What is the difference between reverse transfection and forward transfection? What should I use?

In forward transfection, cells are seeded to appropriate confluence or cell density in wells or dishes, and the lipid-DNA complexes are added the next day. In reverse transfection, the transfection complexes are prepared inside the wells, after which cells and medium are added. Reverse transfection is faster to perform than forward transfection, and is the method of choice for high-throughput transfection. For non-high-throughput transfections, generally forward transfections have better efficiency for most cell types.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Is there a place where I can find references from other researchers who have used your transfection reagents?

Visit the product page for each reagent type and you will see a list of references at the bottom of the page. A table that lists specific cell line references is also accessible. We also recommend www.highwire.org as a search engine to find a large selection of up-to-date research articles using our transfection products. Simply include the name of the transfection reagent and your cell line/application of interest in your search criteria.

Find additional tips, troubleshooting help, and resources within our Transfection Support Center.

Can I use antibiotics in the medium during transfection?

Antibiotics can be used in the medium for culturing of cell lines. However, we do not recommend using antibiotics in the transfection medium unless previously tested in the cell type and payload being transfected. This is because presence of antibiotics during transfection may adversely affect transfection efficiency (i.e., positively charged antibiotics binding to the DNA being transfected) and overall health of cells being transfected.

For stable transfection, we recommend waiting wait 24-48 hrs after transfection before adding selected antibiotics.

Find additional tips, troubleshooting help, and resources within ourTransfection Basics Support Center.

View all Lipofectin™ Transfection Reagent, 4 mL FAQs

Citations & References (54)

Citations & References
Abstract
Transfection techniques for producing recombinant baculoviruses.
Authors:Trotter KM, Wood HA
Journal:Methods Mol Biol
PubMed ID:7620563
A role for calcium in stabilizing transport vesicle coats.
Authors: Ahluwalia J P; Topp J D; Weirather K; Zimmerman M; Stamnes M;
Journal:J Biol Chem
PubMed ID:11435443
'Calcium has been implicated in regulating vesicle fusion reactions, but its potential role in regulating other aspects of protein transport, such as vesicle assembly, is largely unexplored. We find that treating cells with the membrane-permeable calcium chelator, 1,2-bis(2-aminophenoxy)ethane-N,N,N'',N''-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), leads to a dramatic redistribution of the vesicle ... More
Stimulation of p53-mediated transcriptional activation by the p53-binding proteins, 53BP1 and 53BP2.
Authors: Iwabuchi K; Li B; Massa H F; Trask B J; Date T; Fields S;
Journal:J Biol Chem
PubMed ID:9748285
'p53 is a tumor suppressor protein that controls cell proliferation by regulating the expression of growth control genes. In a previous study, we identified two proteins, 53BP1 and 53BP2, that are able to bind to wild type but not to mutant p53 via the DNA-binding domain of p53. We isolated ... More
Hypoxia induces cyclooxygenase-2 via the NF-kappaB p65 transcription factor in human vascular endothelial cells.
Authors: Schmedtje J F Jr; Ji Y S; Liu W L; DuBois R N; Runge M S;
Journal:J Biol Chem
PubMed ID:8995303
'The inducible cyclooxygenase, COX-2, has been associated with vascular inflammation and cellular proliferation. We have discovered that hypoxia increases expression of the COX-2 gene in human vascular endothelial cells in culture independent of other stimuli. Western analysis of human umbilical vein endothelial cells (HUVEC) revealed a greater than 4-fold induction ... More
Expression of the human cystic fibrosis transmembrane conductance regulator gene in the mouse lung after in vivo intratracheal plasmid-mediated gene transfer.
Authors: Yoshimura K; Rosenfeld M A; Nakamura H; Scherer E M; Pavirani A; Lecocq J P; Crystal R G;
Journal:Nucleic Acids Res
PubMed ID:1377820
'As an approach to gene therapy for the respiratory manifestations of cystic fibrosis (CF), in vivo plasmid-mediated direct transfer of the normal CF transmembrane conductance regulator (CFTR) gene to the airway epithelium was investigated in mice. To evaluate the feasibility of this strategy, pRSVL, a plasmid composed of a firefly ... More
View all Lipofectin™ Transfection Reagent, 4 mL citations