Pierce™ DSG, formato No-Weigh™
Pierce™ DSG, formato No-Weigh™
Citas y referencias (6)
Thermo Scientific™

Pierce™ DSG, formato No-Weigh™

Thermo Scientific Pierce DSG es un entrecruzador de éster N-hidroxisucinimida (éster NHS) homobifuncional (NHS-ester) insoluble en agua que se utilizaMás información
Have Questions?
Cambiar vistabuttonViewtableView
Número de catálogoCantidad
2059350 mg
A3539210 x 1 mg
Número de catálogo 20593
Precio (CLP)
148.909
Each
Añadir al carro de la compra
Cantidad:
50 mg
Pedido a granel o personalizado
Precio (CLP)
148.909
Each
Añadir al carro de la compra
Thermo Scientific Pierce DSG es un entrecruzador de éster N-hidroxisucinimida (éster NHS) homobifuncional (NHS-ester) insoluble en agua que se utiliza a menudo para conjugar ligandos radiomarcados con receptores de superficie celular. El éster NHS es el grupo reactivo más simple y más comúnmente utilizado para el entrecruzamiento y marcado de proteínas y péptidos. Los ésteres NHS reaccionan con las aminas primarias en las terminaciones N de los péptidos y la  amina de los residuos de lisina, formando un enlace de amida estable y covalente, y liberando el grupo del NHS.

Los productos Thermo Scientific No-Weigh son reactivos especiales suministrados en un formato previamente indicado. El envase prepesado evita la pérdida de reactividad del reactivo y la contaminación con el tiempo eliminando la apertura y el cierre repetitivos del vial. El formato permite el uso de un vial de reactivo fresco cada vez, lo que elimina la molestia de pesar pequeñas cantidades de reactivos y reduce las preocupaciones sobre la estabilidad del reactivo.

Características del disucinimidilo glutarato:

• Grupos reactivos: Éster NHS (ambos extremos)
• Reactivo a: grupos amino (aminas primarias)
• No escindible
• Insoluble en agua (disolver primero en DMF o DMSO)
• Membrana permeable, permitiendo el entrecruzamiento intracelular
• Puede aumentar la eficiencia de entrecruzamiento en comparación con DSS en algunas aplicaciones

Referencias de producto
Guía de aplicación entrecruzada: búsqueda de referencias bibliográficas recientes para este producto

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Permeabilidad celularSí
FormularioPolvo
Método de etiquetadoEtiqueta química
Peso molecular326.26
PegiladoNo
Línea de productosPierce
Cantidad50 mg
Fracción reactivaÉster NHS
Condiciones de envíoAmbiente
SolubilidadDMF, DMSO
Longitud del brazo del separador7,7 Å
Soluble en aguaNo
Reactividad químicaAmina-amina
CleavableNo
Tipo de enlace cruzadoHomobifuncionales
FormatoEstándar, uso único
Tipo de productoEntrecruzador
SeparadorCorto (< 10 Å)
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar desecado a 4 °C.

Preguntas frecuentes

Can you provide the shelf-life for DSG (disuccinimidyl glutarate)?

DSG (disuccinimidyl glutarate) is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

How should I dissolve and store DSG, No-Weigh Format?

DSG is an NHS-ester that hydrolyzes easily and is moisture-sensitive. For optimal results, we recommend storing DSG powder desiccated at 4 degrees C. To avoid moisture condensation onto the product, the vial must be equilibrated to room temperature before opening. We recommend resuspending DSG in DMSO, Anhydrous (Cat. No. D12345) right before use and not storing the solution.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can NHS-diazirine (Cat. No. 26167) be used for CHIP applications?

NHS-diazirine has not been tested for cross-linking in a ChIP application. EGS (Cat. No. 21565) and DSG (Cat. No. 20593) are not used by themselves in ChIP, but rather in combination with formaldehyde. While formaldehyde is good for crosslinking proteins that are in direct contact with DNA, it cannot trap proteins that may be bound in a complex with other proteins but are not in direct contact. Thus EGS or DSG can cross-link proteins to proteins that are in direct contact with DNA and are crosslinked to it by formaldehyde. See this reference for more information

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Citations & References (6)

Citations & References
Abstract
The structural basis for the oligomerization of the N-terminal domain of SATB1.
Authors:Wang Z, Yang X, Chu X, Zhang J, Zhou H, Shen Y, Long J
Journal:Nucleic Acids Res
PubMed ID:22241778
Special AT-rich sequence-binding protein 1 (SATB1) is a global chromatin organizer and gene expression regulator essential for T-cell development and breast cancer tumor growth and metastasis. The oligomerization of the N-terminal domain of SATB1 is critical for its biological function. We determined the crystal structure of the N-terminal domain of ... More
Neurotrophin-mediated degradation of histone methyltransferase by S-nitrosylation cascade regulates neuronal differentiation.
Authors:Sen N, Snyder SH
Journal:Proc Natl Acad Sci U S A
PubMed ID:22123949
Epigenetic regulation of histones mediates neurotrophin actions with histone acetylation enhancing cAMP response element-binding (CREB)-associated transcription elicited by brain-derived neurotrophic factor (BDNF) and nerve-growth factor (NGF). Roles for histone methylation in CREB's transcriptional activity have not been well characterized. We show that depletion of the histone methyltransferase suppressor of variegation ... More
Characterizing phospholamban to sarco(endo)plasmic reticulum Ca2+-ATPase 2a (SERCA2a) protein binding interactions in human cardiac sarcoplasmic reticulum vesicles using chemical cross-linking.
Authors:Akin BL, Jones LR
Journal:J Biol Chem
PubMed ID:22247554
Chemical cross-linking was used to study protein binding interactions between native phospholamban (PLB) and SERCA2a in sarcoplasmic reticulum (SR) vesicles prepared from normal and failed human hearts. Lys(27) of PLB was cross-linked to the Ca(2+) pump at the cytoplasmic extension of M4 (at or near Lys(328)) with the homobifunctional cross-linker, ... More
A central role for the T1 domain in voltage-gated potassium channel formation and function.
Authors:Strang C, Cushman SJ, DeRubeis D, Peterson D, Pfaffinger PJ
Journal:J Biol Chem
PubMed ID:11312262
To interpret the recent atomic structures of the Kv (voltage-dependent potassium) channel T1 domain in a functional context, we must understand both how the T1 domain is integrated into the full-length functional channel protein and what functional roles the T1 domain governs. The T1 domain clearly plays a role in ... More
RelA Ser276 phosphorylation-coupled Lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection.
Authors:Brasier AR, Tian B, Jamaluddin M, Kalita MK, Garofalo RP, Lu M
Journal:J Virol
PubMed ID:21900162
Respiratory syncytial virus (RSV) is a negative-sense single-stranded RNA virus responsible for lower respiratory tract infections (LRTIs) in humans. In experimental models of RSV LRTI, the actions of the nuclear factor ?B (NF-?B) transcription factor mediate inflammation and pathology. We have shown that RSV replication induces a mitogen-and-stress-related kinase 1 ... More
View all Pierce™ DSG, formato No-Weigh™ citations