Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Thermo Scientific™

Pierce™ Biotinylated Protein Interaction Pull-Down Kit

El kit de «pull-down» de interacción de proteínas biotiniladas Thermo Scientific Pierce incluye los componentes necesarios para capturar y purificarMás información
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Número de catálogoCantidad
211151 kit
Número de catálogo 21115
Precio (CLP)
739.962
Each
Añadir al carro de la compra
Cantidad:
1 kit
Pedido a granel o personalizado
Precio (CLP)
739.962
Each
Añadir al carro de la compra
El kit de «pull-down» de interacción de proteínas biotiniladas Thermo Scientific Pierce incluye los componentes necesarios para capturar y purificar interactores de una proteína o ligando etiquetado con biotina.

Características del kit de «pull-down» de interacción de proteínas biotiniladas:

• Proporciona un sistema completo, asequible y fácil de usar para detectar las interacciones entre proteínas.
• Utiliza reactivos y equipos habituales de laboratorio (por ejemplo, microcentrífugas, minigeles, coloraciones de proteínas).
• Se puede adaptar a las necesidades de una o varias muestras
• El formato flexible de «pull-down» utiliza vasos de centrifugación para una manipulación fácil y eficaz de los gránulos de agarosa y estreptavidina.

Solo tiene que proporcionar la proteína biotinilada que actuará como «cebo» (consulte los

productos relacionados de
EZ-Link™ Kit de «pull-down» y destiobiotinilación).
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoPurificación por afinidad
Para utilizar con (aplicación)Pull-down (basado en biotina)
Para utilizar con (equipo)Microcentrífuga
FormatoKit
Cantidad1 kit
Suficiente para25 reacciones
ObjetivoSin especificidad
Línea de productosPierce
TipoKit de extracción
Unit SizeEach
Contenido y almacenamiento
Suficiente para: Al menos 25 reacciones de «pull-down», cada una con 25 µl de resina
• Resina de agarosa y estreptavidina, 1,5 ml
• 1 paquete de TBS (produce 500 ml)
• Solución de bloqueo de biotina, 15 ml
• Tampón de lavado (pH 5,0), 100 ml
• Tampón de elución (pH 2,8), 50 ml
• 27 columnas de centrifugación Pierce y accesorios
• 200 tubos de recogida para microcentrífugas

Almacenar a 4 °C

Preguntas frecuentes

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.