RPMI 1640 Medium, no glutamine
RPMI 1640 Medium, no glutamine
Citas y referencias (6)
Gibco™

RPMI 1640 Medium, no glutamine

El medio RPMI 1640 se desarrolló originalmente para el cultivo de células leucémicas humanas en suspensión y como monocapa. AMás información
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Número de catálogoCantidad
21870076500 mL
2187009210 × 500 mL
218700841000 mL
218701006 x 1000 mL
Número de catálogo 21870076
Precio (CLP)
-
Cantidad:
500 mL
Customize this product
El medio RPMI 1640 se desarrolló originalmente para el cultivo de células leucémicas humanas en suspensión y como monocapa. A partir de entonces, el medio Roswell Park Memorial Institute (RPMI) 1640 se ha considerado adecuado para una gran variedad de células de mamíferos, incluidas HeLa, Jurkat, MCF-7, PC12, CMSP, astrocitos y carcinomas. Ofrecemos una gran variedad de modificaciones del medio RPMI 1640 para diversas aplicaciones de cultivos celulares. Busque la formulación adecuada mediante la herramienta de selección de medios.

Esta RPMI se ha modificado de la manera siguiente:
ConSin
• Rojo de fenol• Ácido 4-(2-hidroxietil)piperazin-1-iletanosulfónico (HEPES)
• L-glutamina;

Está disponible la formulación completa.

Uso de RPMI
El medio RPMI 1640 es único en comparación con otros medios porque contiene el agente reductor glutatión y altas concentraciones de vitaminas. El medio RPMI 1640 contiene biotina, vitamina B12 y ácido paraaminobenzoico (PABA), que no se encuentran en el medio esencial mínimo de Eagle o el medio Eagle modificado de Dulbecco. Además, las vitaminas inositol y colina están presentes en concentraciones muy elevadas. El medio RPMI 1640 no contiene proteínas, lípidos ni factores de crecimiento. Por lo tanto, el medio RPMI 1640 requiere una suplementación, por lo general con un 10 % de suero fetal bovino (SFB). El medio RPMI 1640 utiliza un sistema de tampones de bicarbonato sódico (2,0 g/l) y, por lo tanto, requiere un ambiente con un 5–10 % de CO2 para mantener el pH fisiológico.

Sistema de fabricación y calidad conforme las prácticas recomendadas de fabricación actuales
El medio RPMI 1640 se fabrica en unas instalaciones conformes con las prácticas recomendadas de fabricación actuales situadas en Paisley, Escocia (Reino Unido). Las instalaciones se han registrado en la Agencia estadounidense de alimentos y medicamentos (FDA) como fabricante de dispositivos médicos y tienen la certificación según la norma ISO 13485. Para la continuidad de la cadena de suministro, podemos ofrecer un producto RPMI 1640 idéntico realizado en nuestras instalaciones de Escocia (31870-074). Estas instalaciones también están registradas en la Agencia estadounidense de alimentos y medicamentos (FDA), calificadas como punto de fabricación de dispositivos médicos y están certificadas según la norma ISO 13485.
Para su uso en investigación o procesos de fabricación posteriores. No apto para uso diagnóstico ni para la administración directa en seres humanos ni en animales.
Especificaciones
Línea de célulasHeLa, Jurkat, MCF-7, PC-12, PBMC, astrocitos y carcinomas
Tipo de célulaCélulas leucémicas
Concentración1 X
Calidad de fabricacióncGMP-compliant under the ISO 13485 standard
Línea de productosGibco
Tipo de productoMedio RPMI 1640 (medio Roswell Park Memorial Institute 1640)
Cantidad500 mL
Duración de almacenamiento12 meses a partir de la fecha de fabricación
Condiciones de envíoTemperatura ambiente
ClasificaciónLibre de material de origen animal
FormularioLíquido
Serum LevelSuplementos de suero estándar
EsterilidadEstéril con filtro
Sterilization MethodEstéril con filtro
Con aditivosRojo de fenol
Sin aditivosSin glutamina, Sin HEPES, Sin piruvato sódico
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 2 a 8 °C. Proteger de la luz
Condiciones de envío: Ambiente
Vida útil: 12 meses a partir de la fecha de fabricación

Preguntas frecuentes

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Medio RPMI 1640, sin glutamina FAQs

Citations & References (6)

Citations & References
Abstract
Fatty acids regulate stress resistance and virulence factor production for Listeria monocytogenes.
Authors:Sun Y, Wilkinson BJ, Standiford TJ, Akinbi HT, O'Riordan MX
Journal:J Bacteriol
PubMed ID:22843841
'Fatty acids (FAs) are the major structural component of cellular membranes, which provide a physical and chemical barrier that insulates intracellular reactions from environmental fluctuations. The native composition of membrane FAs establishes the topological and chemical parameters for membrane-associated functions and is therefore modulated diligently by microorganisms especially in response ... More
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O.
Authors:Walev I, Bhakdi SC, Hofmann F, Djonder N, Valeva A, Aktories K, Bhakdi S,
Journal:Proc Natl Acad Sci U S A
PubMed ID:11248053
'The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10(5)-10(6) molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca(2+)-calmodulin and on ... More
Novel in vitro and in vivo models and potential new therapeutics to break the vicious cycle of Cryptosporidium infection and malnutrition.
Authors:Costa LB, Noronha FJ, Roche JK, Sevilleja JE, Warren CA, Oriá R, Lima A, Guerrant RL
Journal:J Infect Dis
PubMed ID:22454464
Although several animal models of cryptosporidiosis have been reported, most involve genetically or pharmacologically immune-suppressed hosts.
A novel zinc-regulated human zinc transporter, hZTL1, is localized to the enterocyte apical membrane.
Authors: Cragg Ruth A; Christie Graham R; Phillips Siôn R; Russi Rachel M; Küry Sébastien; Mathers John C; Taylor Peter M; Ford Dianne;
Journal:J Biol Chem
PubMed ID:11937503
Zinc is essential to a wide range of cellular processes; therefore, it is important to elucidate the molecular mechanisms of zinc homeostasis. To date, no zinc transporters expressed at the enterocyte apical membrane, and so essential to mammalian zinc homeostasis, have been discovered. We identified hZTL1 as a human expressed ... More
Regulation of Fas-associated death domain interactions by the death effector domain identified by a modified reverse two-hybrid screen.
Authors: Thomas Lance R; Stillman David J; Thorburn Andrew;
Journal:J Biol Chem
PubMed ID:12107169
The adapter protein FADD consists of two protein interaction domains and is an essential component of the death inducing signaling complex (DISC) that is formed by activated death receptors of the tumor necrosis factor (TNF) receptor family. The FADD death domain binds to activated receptors such as Fas or other ... More
View all Medio RPMI 1640, sin glutamina citations