Suero fetal bovino, cualificado, Estados Unidos
Suero fetal bovino, cualificado, Estados Unidos
Gibco™

Suero fetal bovino, cualificado, Estados Unidos

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust ofMás información
Have Questions?
Número de catálogoCantidad
261400951000 mL
Número de catálogo 26140095
Precio (CLP)
1.936.216
Each
Añadir al carro de la compra
Cantidad:
1000 mL
Precio (CLP)
1.936.216
Each
Añadir al carro de la compra

Gibco fetal bovine sera offer excellent value for basic cell culture, specialty research, and specific assays, earning the trust of researchers with consistent quality and award-winning support that helps meet your research needs and budget requirements

Gibco Sera: Trusted Quality for Consistent Performance

Gibco Sera Category: Value Plus (Performance)

  • Use for general cell culture with common cell lines
  • Low endotoxin and hemoglobin levels
  • Endotoxin level: <10 EU/mL
  • Hemoglobin level: <25 mg/dL
  • Origin: United States

Gibco Serum Delivers

  • ISO 13485 certified, processed in FDA registered facilities
  • Triple filtered at 0.1 μm
  • Gibco bottle is easier to use in the hood, reduces the risk of contamination and helps you perform cell culture more consistently
  • Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Especificaciones
Concentración de endotoxinas≤10 EU/mL
Concentración de hemoglobina≤25 mg/dL
Grado de pureza o calidadCualificado
Condiciones de envíoFrozen
EspecieCattle/Bovine
AgeFetal
País de origenEstados Unidos
FormularioLíquido
FormatoBottle
Tipo de productoFetal Bovine Serum
Cantidad1000 mL
Serum TreatmentStandard (Sterile-filtered)
EsterilidadEstéril
Sterilization MethodTriple-filtered, 0.1 μm
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: ≤10 °C
Condiciones de envío: Congelado

Preguntas frecuentes

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (1)

Citations & References
Abstract
Raloxifene improves endothelial dysfunction in hypertension by reduced oxidative stress and enhanced nitric oxide production.
Authors: Wassmann Sven; Laufs Ulrich; Stamenkovic Djordje; Linz Wolfgang; Stasch Johannes-Peter; Ahlbory Katja; Rösen Renate; Böhm Michael; Nickenig Georg;
Journal:Circulation
PubMed ID:11980689
BACKGROUND: It has not been completely clarified whether selective estrogen receptor modulators (SERMs) such as raloxifene exert vasoprotective effects similar to those of estrogens. METHODS AND RESULTS: To investigate vascular effects of raloxifene, male spontaneously hypertensive rats were treated for 10 weeks with either raloxifene (10 mg x kg(-1) x ... More