Estándar de proteínas de rango bajo multicolor Spectra™
Estándar de proteínas de rango bajo multicolor Spectra™
Thermo Scientific™

Estándar de proteínas de rango bajo multicolor Spectra™

La escalera de proteínas de rango bajo multicolor Thermo Scientific Spectra es una mezcla de seis proteínas y péptidos sintéticosMás información
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Número de catálogoCantidad
26628250 μl
Número de catálogo 26628
Precio (CLP)
188.119
Each
Añadir al carro de la compra
Cantidad:
250 μl
Pedido a granel o personalizado
Precio (CLP)
188.119
Each
Añadir al carro de la compra
La escalera de proteínas de rango bajo multicolor Thermo Scientific Spectra es una mezcla de seis proteínas y péptidos sintéticos (de 1,7 kDa a 40 kDa) que se han diseñado para usar como patrones de tamaño para péptidos y proteínas pequeñas en electroforesis en gel e inmunotransferencia (Western blotting). La escalera de proteínas se suministra en un formato listo para usar para la carga directa en geles; sin necesidad de calentar, reducir o añadir el tampón de muestra antes de su uso.

Compare y visualice todos los demás patrones y escaleras de proteínas ›

Aplicaciones
• Supervisión de la migración de proteínas durante la SDS-PAGE
• Verificación de la eficiencia de transferencia Western
• Dimensionamiento aproximado de proteínas en geles de poliacrilamida-SDS e inmunotransferencia (Western blot)
• Localización de una proteína de interés para la escisión de un gel preparativo no teñido

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescencia colorimétrica, NIR (700 nm), fluorescencia RGB (555 nm)
Para utilizar con (aplicación)Tricine gels
Compatibilidad del gelGeles Bolt™ Bis-Tris Plus, Geles de tricina Novex™, geles de Tris-glicina Novex™, geles NuPAGE™ Bis-Tris, geles de Tris-acetato NuPAGE™, geles SDS-PAGE
Peso molecular40, 25, 15, 10, 4,6, 1,7 kDa
Línea de productosSpectra
Tipo de productoMarcadores moleculares de proteínas
Cantidad250 μl
Listo para cargar
Aplicaciones recomendadasGeles de tricina
Condiciones de envíoAprobado para su envío en hielo húmedo o seco
Tipo de tinciónTres colores: Azul, naranja, verde
Tipo de sistemaWestern Blotting, SDS-PAGE
Number of Markers6
Intervalo de tamañosDe 1,7 a 40 kDa
Unit SizeEach
Contenido y almacenamiento
Contenido: un vial de 250 μl

Tampón de almacenamiento: 62,5 mM Tris-H3PO4 (pH 7,5 a 25 °C), 1 mM EDTA, 2 % SDS, 10 mM DTT, 1 mM NaN3 y 33 % glicerol

Almacenamiento: Tras su recepción, almacenar a -20 °C

Preguntas frecuentes

Why do Thermo Scientific prestained protein ladders not show the real protein sizes?

Coupling of chromophores to proteins affects the apparent molecular weight of proteins in SDS-PAGE relative to unstained standards. The apparent molecular weight of prestained protein standards is calibrated in the classical TRIS glycine-SDS Laemmli system, however prestained proteins may have different mobility in other electrophoresis buffer and gel systems. It should also be noted that the sizing of proteins by gel electrophoresis does not give an exact value and depends on the protein sequence and post-modification.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The upper bands of the ladder are missing. What could be the reason?

The upper bands of the ladder may be degraded by proteases. Ladder, gel, buffer, pipettes, pipette tips, or equipment can be contaminated by proteases during usage. A general recommendation would be to avoid working with proteases in the same room. We would recommend preparing fresh solutions, cleaning the equipment, and using clean pipettes and tips. If the ladder itself is contaminated, please use a new tube of the ladder.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Do the proteins in Thermo Scientific protein ladders have a His-Tag or would otherwise react with an anti-His-Tag antibody?

No, proteins in Thermo Scientific protein ladders are not His tagged. However, non-specific interaction between the ladder proteins and primary or secondary antibodies is possible and some His-Tag detection systems, such as Thermo Scientific 6xHis Protein Tag Stain Reagent Kit, show non-specific interaction. The protein ladder bands are more readily detected when using high antibody concentrations. The non-specific cross-reactivity is difficult to predict, it often has a different pattern dependent on the antibodies used in each individual experiment. The most general way to handle this problem would be to use lower concentrations of antibodies and to use lower amount of protein ladders. It may also be useful to leave one empty well between the ladder and the sample to overcome a possible leakage of the signal to the nearby sample lane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can Thermo Scientific protein ladders be detected by Strep-Tactin conjugates?

PageRuler Unstained protein ladders can be detected directly on Western blots by using Strep-Tactin conjugates or an antibody against the Strep-tag II sequence. All PageRuler and Spectra ladder proteins contain an integral Strep-tag II sequence, however the prestained ladders cannot be detected by Strep-Tactin conjugates.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Why are the PageRuler and Spectra ladder bands detected with antibodies against eukaryotic proteins?

All PageRuler and Spectra ladder bands are recombinant prokaryotic proteins purified from E. coli cells. None of them are related to eukaryotic proteins, however this cannot exclude the possibility that the ladder proteins may possess an epitope that is cross-reactive with the antibody used.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.