Pierce™ Immunodiffusion Plates, Multiple Pattern
Pierce™ Immunodiffusion Plates, Multiple Pattern
Thermo Scientific™

Pierce™ Immunodiffusion Plates, Multiple Pattern

Las placas de inmunodifusión Thermo Scientific Pierce son placas de gel Ouchterlony estándar que contienen patrones de seis pocillos alrededorMás información
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Número de catálogoCantidad
311116 plates
Número de catálogo 31111
Precio (CLP)
-
Cantidad:
6 plates
Las placas de inmunodifusión Thermo Scientific Pierce son placas de gel Ouchterlony estándar que contienen patrones de seis pocillos alrededor de un pocillo para estudiar y caracterizar las interacciones de unión entre anticuerpos y antígenos.

Características de las placas de inmunodifusión Pierce:

• El agente gelificante contiene iluminadores de precipitina para facilitar la visualización.
• Las placas de inmunodifusión contienen potenciadores de difusión para ayudar a acelerar el proceso de interacción.
• Se proporcionan bandas de precipitina excelentes con anticuerpos de todas las especies (incluidos los anticuerpos de conejo).
• Los geles se pueden lavar, secar y teñir para obtener un registro permanente.

La inmunodifusión (ID) es una técnica clásica para detectar interacciones entre anticuerpos y antígenos que se basa en la teoría de la doble difusión descrita originalmente por Oudin y Ouchterlony. Los antígenos y los anticuerpos se colocan en pocillos separados que se cortan en una matriz de gel y se deja que se produzca difusión entre estos. Si la reacción es positiva, se forma un precipitado que aparece como una línea opaca. La reacción de precipitación ocurre cuando las concentraciones de antígeno y anticuerpo se combinan en proporciones casi equivalentes.

Cuando los antígenos multivalentes se combinan con anticuerpos divalentes en solución, se forman retículas tridimensionales que se agregan y precipitan. La cantidad de precipitado varía en proporción a la concentración del antígeno y el anticuerpo. En proporciones equivalentes u óptimas, casi todos los antígenos y anticuerpos se precipitarán. Si hay exceso de anticuerpos, los complejos que se forman con el antígeno son insolubles. Cuando hay exceso de antígenos, el precipitado tiene tendencia a disolverse debido a la formación de complejos solubles.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
ensayoInmunodifusión (ID)
Método de detecciónPrecipitado
Línea de productosPierce
Cantidad6 plates
Especificidad de dianaSin especificidad de elemento objetivo
TécnicaInmunodifusión (ID)
TipoPlaca multipocillo
Volumen de trabajo (métrico)22 μl
ColorTransparente
Diámetro (métrico)3,2mm
Altura (métrico)2,8mm
Número de pocillos6
Unit SizeEach
Contenido y almacenamiento
Tras su recepción, almacenar a 4 °C. Las placas se envían a temperatura ambiente. No congele las placas ni permita que se sequen.

Preguntas frecuentes

What do I need to consider when designing a peptide for antibody production?

We recommend that hydrophobic residues comprise 50% or less of all the residues in your sequence. Make sure there is at least one charged residue for every five amino acids: this is generally known to enhance the solubility of the peptide. Peptides (compared to polypeptides, which fold and bury the hydrophobic amino acids) are too small to fold, so just a few hydrophobic amino acids may leave them insoluble.

Other guidelines include:
Peptides containing multiple Cys, Met, and Trp can be hard to synthesize.
Some sequences are problematic in solid-phase peptide synthesis or cleavage and are best avoided; these include Asp-Pro sequences or stretches of amino acids that require bulky protecting groups on their side chains during synthesis.
Glycine is often good for antigenicity as it has only a hydrogen side chain; this allows for complete rotation.
Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What is the optimal peptide length for antibody production?

Most peptide antigens range in length from 12 to 16 residues and are relatively easy to synthesize. Peptides of 9 residues or shorter have been effective antigens for antibody production, but peptides longer than 16 amino acids may contain several epitopes and form secondary structures. Peptides in excess of 18 residues begin to present more synthetic challenges. Before you synthesize your peptide, we recommend doing a BLASTP search using your peptide sequence. This is to make sure that the peptide is not homologous or identical to a sequence in a completely unrelated protein in the host animal.

Please note that we offer a Custom Antibody Production service (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production.html) that includes the use of our proprietary Antigen Profiler and Antigen Preparation tool (https://www.thermofisher.com/us/en/home/life-science/antibodies/custom-antibodies/custom-antibody-production/antigen-profiler-antigen-preparation.html).

What happens if antibodies are stored improperly?

Improper storage of antibodies can lead to:
- Degradation: Loss of activity and specificity
- Aggregation: Formation of precipitates or clumps
- Reduced performance: Poor results in assays or experiments

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do antibody pair kits contain, how many samples can I process, and how can I find a list of antibody pair kits by target?

Antibody pair kits contain capture antibody, detection antibody, recombinant standard and HRP conjugate. Each contains enough reagents to process forty 96-well plates. A list of Antibody Pair Kits (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) is available by target.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.

What do Reagent Sets contain, how many samples can I process, and how can I check if a Reagent Set is available for my target?

Reagent Sets contain Capture Antibody, Detection Antibody, Recombinant Standard, HRP Conjugate, TMB Substrate and Stop Solution. Each contains enough reagents to process five 96-well plates. Reagent Sets are included in the main list of ELISA (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/elisa/antibody-pair-kits.html) kits (search by “Reagent Set”).

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.