TaqMan™ Sample-to-SNP™ Kit (200 mL sample prep, 10 mL PCR)
Product Image
Applied Biosystems™

TaqMan™ Sample-to-SNP™ Kit (200 mL sample prep, 10 mL PCR)

The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than oneMás información
Have Questions?
Número de catálogoCantidad
44030811 kit
Número de catálogo 4403081
Precio (CLP)
6.444.539
Each
Añadir al carro de la compra
Cantidad:
1 kit
Precio (CLP)
6.444.539
Each
Añadir al carro de la compra
The Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hr. The TaqMan™ Sample-to-SNP™ Kit combines DNA extraction and TaqMan™ chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan™™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems™ TaqMan™ Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 4,000 sample preparation reactions at 50 μL/ reaction and 4,000 PCR reactions at 5 μL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan™ Sample-to-SNP™ Kit.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Para utilizar con (equipo)Sistema 7300, sistema 7500 Fast, sistema 7500, sistema 7900HT Fast, GeneAmp 9700, StepOne™, modo rápido, StepOne™, modo estándar, termociclador Veriti, StepOne™ modo estándar,StepOne™ modo rápido
Objetivo de genotipadoSNP (desconocidos o numerosos), SNP (conocidos)
Inicio en calienteBuilt-In Hot Start
N.º de reacciones4000 Reactions (5 μL, PCR), 4000 Reactions (50 μL/Reaction, Sample Preparation)
Colorante de referencia pasivaROX (premezclado)
PolimerasaAmpliTaq DNA Polymerase
Línea de productosSample-to-SNP, TaqMan
Tipo de productoPCR Kit
Cantidad1 kit
Tipo de muestraMuestra bucal, cultivos celulares, cabello con folículo, perforación de hoja o aguja, viruta de semilla, biopsia de cola (ratón), biopsia de cola (rata), tejido (FFPE), sangre completa
TécnicaSNP Genotyping (Real-Time PCR-Based)
Volumen10 mL (PCR), 200 mL (Sample Preparation)
Método de detecciónSonda de cebado
Método de PCRqPCR
Velocidad de reacciónrápida o estándar
Unit SizeEach
Contenido y almacenamiento
Contains Lysis Reagents (2 -8°C): 200 mL each Lysis and DNA Stabilization Solution; Master Mix (15 to 25oC): 10 mL 2X master mix with AmpliTaq™ Fast DNA Polymerase, UP, dNTPs, Tracking dye and ROX™ Dye.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

Preguntas frecuentes

When designing probes to perform Allelic Discrimination (SNP assays), are there any alternatives to using probes longer than 30bp in order to get the appropriate melting temperature?

Applied Biosystems TaqMan MGB (minor groove binder) probes would be appropriate for Allelic Discrimination/SNP Genotyping probe designs. TaqMan MGB probes have a minor groove binder at the end of the probe. This minor groove binder increases the Tm of probes, allowing the use of shorter probes. Consequently, the TaqMan MGB probes exhibit greater differences in Tm values between matched and mismatched probes, which provides more accurate allele discrimination.

For information on the design of TaqMan MGB probes for Allelic Discrimination/SNP assays using Primer Express software, please refer to our guide "Designing TaqMan MGB Probe and Primer Sets for Allelic Discrimination Assays Using Primer Express".