PrepSEQ™ Residual DNA Sample Preparation Kit

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures ofMás información

Número de catálogo	Cantidad
4413686	100 preparaciones

Número de catálogo 4413686

Precio (CLP)

1.210.100

Añadir al carro de la compra

Cantidad:

100 preparaciones

Precio (CLP)

1.210.100

Añadir al carro de la compra

The Applied Biosystems PrepSEQ Residual DNA Sample Preparation Kit is optimized for highly efficient DNA recovery from complex mixtures of proteins, buffers, and salts which are typical of samples that need to be quantitated for residual host cell line DNA. Quantitative and consistent recovery of DNA can be obtained from challenging matrices, including samples that have high protein concentration, low pH, or high salt.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de producto finalADN genómico

Para utilizar con (aplicación)Impurity Testing

Compatibilidad de alto rendimientoNot High-throughput Compatible (Manual), Automated Protocols

N.º de reacciones100 preps

Línea de productosPrepSEQ

Cantidad100 preparaciones

Tipo de muestraBiopharmaceutical Production Samples

Volumen de muestra100 μL

BásculaMini

ObjetivoADN genómico

Tiempo de prueba2 hr.

Isolation TechnologyMagnetic Bead

Unit SizeEach

Contenido y almacenamiento

Includes buffers, magnetic beads and enzymes for DNA extraction for residual DNA quantitation for 100 reactions

Preguntas frecuentes

The magnetic beads, that are shipped with the PrepSeq Residual DNA Sample Preparation Kit (Cat. No. 4413686), are clumped together and are difficult to get back into suspension. How can I fix this?

The beads are known to clump due to precipitation of the salts in the solution. These are highly concentrated chaotropic salt solutions. The only way to get the salts and the beads back into the solution is by incubating them at 37 degrees C for at least 10 minutes or until the solution is homogenous. Intermittent high speed vortexing helps expedite the process. To get beads stuck to the lid back into solution, we recommend inverting the tube and using high speed vortexing in a pulsing manner.
If incubating at 37 degrees C does not fix the issue, we recommend incubating at 45 degrees C.

What amount of DNA can be recovered with the PrepSEQ Residual DNA Sample Preparation Kit (Cat. No. 4413686)?

With the above kit, up to 5 ng of DNA can be recovered per 30 µl magnetic beads used per reaction. PrepSEQ is specially designed to recover lower amounts of DNA (in ng to sub femto‐gram levels).