Applied Biosystems™

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

Número de catálogo: 4426715
Applied Biosystems™

PrepSEQ™ Rapid Spin Sample Preparation Kit - Extra Clean with Proteinase K

Número de catálogo: 4426715
Número de catálogo
4426715
Tamaño de la unidad
1 kit
Número de catálogoTamaño de la unidadPrecio (CLP)
44267151 kitSolicite un presupuesto
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The PrepSEQ™ Spin Sample Preparation Kit – Extra Clean with Proteinase K is recommended for difficult to lyse bacteria, like Listeria monocytogenes, that also has a high lipid content, such as soft cheeses. The kit was developed for the rapid preparation of DNA from food and environmental samples for use in PCR amplification reactions. Using a simple spin protocol, PrepSEQ™ Rapid Spin efficiently inactivates PCR inhibitors. For laboratories involved in food and environmental testing, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. This has created the demand for a sample preparation method that is fast, cost-effective and capable of extracting high-quality DNA from a broad range of sample types. While such methods have been developed for dedicated, high-throughput laboratories, there is a growing need for methods suitable to low-to-medium throughput applications. The PrepSEQ™ Rapid Spin Kit – Extra Clean with Proteinase K meets this need.
• Designed for Food Safety and Environmental Monitoring applications
• Supports low-to-medium throughput work loads (up to 100 samples a day)
• Inactivates PCR inhibitors so you will most likely not have to repeat the template preparation step
• Uses a simple spin protocol
Optimized for PCR-based Food Safety and Environmental Monitoring
For laboratories involved in food safety and environmental monitoring, PCR-based methods are rapidly becoming the standard for accurate, sensitive bacterial detection. Use this sample preparation method for fast, cost-effective extraction of high-quality DNA from a broad range of sample types including chocolate, soft cheeses, and soil. Even samples with difficult-to-lyse bacteria, such as Listeria monocytogenes, produce excellent results with the addition of a Proteinase K digestion step.

Designed for Laboratories that Prepare up to 100 Samples a Day
Kits exist for dedicated, high-throughput laboratories, but now you have a kit that is suitable for low-to-medium throughput applications. This kit is designed for laboratories that routinely prepare up to 100 samples a day.

Removes PCR Inhibitors
The PrepSEQ™ Rapid Spin Sample Preparation Kit offers a simple, cost-effective way to prepare bacterial DNA from broth cultures. During the extraction process, the media inside the spin column removes PCR inhibitors from the sample, allowing the bacteria to pass through into the sample collection tube where it can be stored or used for PCR.


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For Research Use Only. Not for use in diagnostics procedures.

Especificaciones

Formato
Spin Column
Isolation Technology
Spin Column, Coarse Filtration, Enzymatic
Tipo de muestra
Swab, Bacteria (Gram +/-), Bacterial Culture, Bacteria (Hard-to-Lyse), Food (High Lipid Content), Water
Tipo de producto final
Bacterial DNA
Para utilizar con (equipo)
Centrifuge
Para utilizar con (aplicación)
Real-Time Quantitative PCR (qPCR), PCR
Compatibilidad de alto rendimiento
Not High-throughput Compatible (Manual)
N.º de reacciones
100 Reactions
Línea de productos
PrepSEQ™
Objetivo
Genomic DNA
Cantidad de material de partida
750 μL

Contenido y almacenamiento

Each kit contains 100 spin columns, 200 microcentrifuge tubes, Lysis Buffer (store at 4°C), and Proteinase K (store at -20°C). 100 samples can be prepared using this kit.

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